Detection of qnr genes among Escherichia coli isolates of animal origin and complete sequence of the conjugative qnrB19-carrying plasmid pQNR2078

The Journal of Antimicrobial Chemotherapy
Anne-Kathrin SchinkStefan Schwarz

Abstract

The aims of this study were to identify qnr genes among quinolone-resistant Escherichia coli isolates from defined disease conditions of companion and farm animals obtained in the BfT-GermVet study, and to gain insight into their localization and the organization of the qnr gene regions. The qnr genes were detected by PCR and confirmed by sequencing. qnr-positive isolates were checked for mutations in DNA gyrase and topoisomerase IV genes by PCR and sequencing of the quinolone resistance-determining regions. Multilocus sequence typing (MLST) was performed for the qnr-positive E. coli isolates. Plasmids harbouring qnr genes were transferred by conjugation into E. coli recipients, subjected to PCR-based replicon typing and plasmid-MLST, and one qnrB19-carrying plasmid was sequenced completely. Only 2 of 417 E. coli isolates investigated carried qnr genes. Both isolates originated from horses and showed MLST type ST86. They harboured conjugative qnrB19-carrying plasmids, which proved to be indistinguishable by restriction analysis, belonged to incompatibility group IncN, showed plasmid-MLST type ST8 and did not carry other resistance genes. The qnrB19 gene was flanked by copies of the insertion sequence IS26. One of these plasmids...Continue Reading

References

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Jun 21, 2011·Applied and Environmental Microbiology·Anne-Kathrin SchinkStefan Schwarz

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Citations

Jun 19, 2015·Equine Veterinary Journal·T W MaddoxG L Pinchbeck
Jul 11, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·María Paula QuirogaDaniela Centrón
Sep 24, 2016·The Journal of Antimicrobial Chemotherapy·Stefan SchwarzEngeline van Duijkeren
Jan 6, 2019·Applied and Environmental Microbiology·Yongqiang YangRodrigo C Bicalho
Jul 2, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Robab AzargunShirin Eyvazi
Jan 15, 2015·Microbiology Spectrum·George A JacobyDavid C Hooper
Jul 14, 2018·Microbiology Spectrum·Laurent PoirelStefan Schwarz

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