Detection of rabies virus antigen by the indirect rapid immunohistochemistry test in equines and comparisons with other diagnostic techniques.

Zoonoses and Public Health
Ranieli BezerraColletti TorquatoFernanda Guedes

Abstract

Laboratory diagnosis of rabies in equines is essential for distinguishing the disease from other sources of encephalitis. Diagnosis by conventional techniques such as a direct fluorescent antibody test (dFAT) or viral isolation in mice or cell culture can be difficult, and the application of molecular biological methods may be necessary. We performed an indirect rapid immunohistochemistry test (iRIT) for the detection of the rabies virus (RABV) antigen in the central nervous system (CNS) of equines and compared the results with those of other diagnostic techniques. We reviewed result records from the Rabies Diagnosis Laboratory at Instituto Pasteur, São Paulo, Brazil, of 174 samples of equine CNS from July 2014 to June 2016, which were investigated by dFAT, rabies tissue culture infection test (RTCIT), mouse inoculation test (MIT) and reverse transcription-polymerase chain reaction (RT-PCR) followed by genetic sequencing. These samples, 29 presented divergent results among techniques and were selected for the performed in the iRIT. The detected positivity rate was 4/29 (14%) by dFAT, 5/28 (18%) by RTCIT, 10/29 (35%) by MIT and 26/27 (96%) by RT-PCR. We analysed 29 samples through imprints of the cortex, hippocampus, cerebellum ...Continue Reading

References

Feb 24, 2006·Emerging Infectious Diseases·Tiziana LemboCharles E Rupprecht
Aug 22, 2006·Journal of Virological Methods·Maria Luiza CarrieriPedro Manuel Leal Germano
Mar 28, 2008·PLoS Neglected Tropical Diseases·Salome DürrJakob Zinsstag
Sep 30, 2009·PLoS Neglected Tropical Diseases·Anthony R FooksThomas Müller
Sep 3, 2010·Journal of Clinical Microbiology·Jessica CoertseWanda Markotter
Oct 12, 2012·Virologica Sinica·Shampur Narayan MadhusudanaYajaman Belludi Ashwin
Apr 2, 2013·Journal of Virological Methods·Jessie L DyerCharles E Rupprecht
Dec 19, 2013·TheScientificWorldJournal·Reeta Subramaniam Mani, Shampur Narayan Madhusudana
Jun 30, 2017·Tropical Medicine and Infectious Disease·Kevin MiddelTore Buchanan
Jul 5, 2019·Epidemiologia E Serviços De Saúde : Revista Do Sistema Unico De Saúde Do Brasil·Juliana Amorim UbaranaSantuzza Arreguy Silva Vitorino

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