Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction.

Analytical Chemistry
T MatsunagaN Nakamura

Abstract

Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run in the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.65 V vs SCE. There is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1. In the range of (0.4-2.0) X 10(5) cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

References

Sep 1, 1987·Clinical Allergy·J Merrett, T G Merrett
Jan 1, 1987·International Archives of Allergy and Applied Immunology·B J WalshM E Howden
Sep 1, 1987·Clinical Allergy·Y Green-Graif, P W Ewan
Feb 1, 1972·Japanese Journal of Pharmacology·K SaekiH Yamasaki
Apr 1, 1984·Analytical Chemistry·T Matsunaga, Y Namba
Jan 1, 1980·Journal of Immunological Methods·K A KellyA H Sehon

❮ Previous
Next ❯

Citations

Aug 1, 1995·Applied Microbiology and Biotechnology·N NakamuraT Matsunaga
May 17, 2011·Journal of Biological Engineering·Gusphyl A JustinRobert J Sclabassi
May 26, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jia LiShouzhuo Yao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.