Detection of recurrent copy number loss at Yp11.2 involving TSPY gene cluster in prostate cancer using array-based comparative genomic hybridization

Cancer Research
Sapna VijayakumarSusan L Naylor

Abstract

Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of the prostate cancer cell line PC-3. To further characterize the Y chromosome, we have developed a high-density bacterial artificial chromosome (BAC) microarray containing 178 BAC clones from the human Y chromosome. BAC microarray was used for array comparative genomic hybridization on prostate cancer samples and cell lines. The most prominent observation on prostate cancer specimens was a deletion at Yp11.2 containing the TSPY tandem gene array. Out of 36 primary prostate tumors analyzed, 16 (44.4%) samples exhibited loss of TSPY gene copies. Notably, we observed association between the number of TSPY copies in the blood and the incidence of prostate cancer. Moreover, PC-3 hybrids with an intact Yp11.2 did not grow tumors in nude mice, whereas PC-3 hybrids with a deletion at Yp11.2 grew tumors in nude mice.

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Citations

Apr 6, 2013·Journal of Animal Science and Biotechnology·Ayan MukherjeeSachinandan De
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chihiro AkimotoShigeaki Kato
Jan 6, 2011·Systems Biology in Reproductive Medicine·Yun-Fai Chris LauTatsuo Kido
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Dec 21, 2012·Journal of Proteome Research·Zohreh JangraviGhasem Hosseini Salekdeh

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