Detection of Resistance to Fluoroquinolones and Second-Line Injectable Drugs Among Mycobacterium tuberculosis by a Reverse Dot Blot Hybridization Assay

Infection and Drug Resistance
Gui Lian LiKang-Lin Wan

Abstract

Reliable and timely determination of second-line drug resistance is essential for early initiation effective anti-tubercular treatment among multi-drug resistant (MDR) patients and blocking the spread of MDR and extensively drug-resistant tuberculosis. Molecular methods have the potency to provide accurate and rapid drug susceptibility results. We aimed to establish and evaluate the accuracy of a reverse dot blot hybridization (RDBH) assay to simultaneously detect the resistance of fluoroquinolones (FQs), kanamycin (KN), amikacin (AMK), capreomycin (CPM) and second-line injectable drugs (SLIDs) in Mycobacterium tuberculosis. We established and evaluated the accuracy of the RDBH assay by comparing to the phenotypic drug susceptibility testing (DST) and sequencing in 170 M. tuberculosis, of which 94 and 27 were respectively resistant to ofloxacin (OFX) and SLIDs. The results show that, compared to phenotypic DST, the sensitivity and specificity of the RDBH assay for resistance detection were 63.8% and 100.0% for OFX, 60.0% and 100.0% for KN, 61.5% and 98.1% for AMK, 50.0% and 99.3% for CPM, and 55.6% and 100% for SLIDs, respectively; compared to sequencing, the sensitivity and specificity of the RDBH assay were 95.2% and 100.0% f...Continue Reading

References

Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Courtney E MausThomas M Shinnick
Feb 12, 2009·Journal of Biosciences·Amita Jain, Pratima Dixit
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·M Analise ZaunbrecherJames E Posey
Sep 10, 2010·The New England Journal of Medicine·Catharina C BoehmeMark D Perkins
Feb 24, 2012·The Journal of Antimicrobial Chemotherapy·Frederick A SirgelErik C Böttger
Jun 8, 2012·The New England Journal of Medicine·Yanlin ZhaoYu Wang
Dec 20, 2013·Journal of Clinical Microbiology·Timothy C RodwellAntonino Catanzaro
Jan 15, 2014·Antimicrobial Agents and Chemotherapy·Li-Li ZhaoKang-Lin Wan
Jun 8, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Z ZhangY Zhao
Sep 6, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·C ChenW Lu
Dec 18, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·J FurinK Kielmann
Oct 16, 2015·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·P KambliC Rodrigues
Nov 26, 2015·The Journal of Antimicrobial Chemotherapy·L RigoutsA Van Deun
Dec 22, 2015·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·J DomínguezUNKNOWN RESIST-TB networks
Aug 19, 2016·BMC Infectious Diseases·Evangelina Inácio NambureteValdes Roberto Bollela
Oct 23, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D PapaventsisV Nikolayevskyy
Jul 19, 2017·Biologicals : Journal of the International Association of Biological Standardization·Fatemeh SakhaeeSeyed Davar Siadat
Mar 28, 2019·Infectious Disorders Drug Targets·Eltayib H Ahmed AbakurKhalid A S Alfifi
Aug 6, 2019·American Journal of Respiratory and Critical Care Medicine·Heather J ZarMark P Nicol
Aug 16, 2019·Journal of Clinical Microbiology·Kamela C S NgLeen Rigouts
Jan 15, 2020·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R J ChandakJ Tiwari
Jan 25, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Fengmin HuoYu Pang
Feb 27, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Vangala RamakrishnaAmita Jain

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Methods Mentioned

BETA
dot
PCRs
PCR
electrophoresis
Assay
dot blot

Software Mentioned

BLAST
Primer Premier
GenoType
SPSS

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