Abstract
Nasal aspirates from 238 infants hospitalized with acute respiratory infections during the winter of 1994 and 1995 were tested for respiratory syncytial virus (RSV) by immunofluorescence assay (IFA) and the viral isolation technique (VIT) and by two PCR and hybridization methods: reverse transcription PCR 1 (RT-PCR1), which amplifies the RNAs of all RSV strains, and RT-PCR-2, which allows subgroup classification of RSV. RT-PCR-1 and RT-PCR-2 detected viral sequences in 56.7% (135 of 238) and 48.3% (115 of 238) of the samples, respectively, while only 80 (33.6%) samples were found to be positive by IFA and VIT. Of the PCR-positive specimens, 57 were missed by these routine techniques in RT-PCR-1 and 45 were missed in RT-PCR-2. Although the RSV-PCR-1 and RSV-PCR-2 techniques amplified two different sequences of the RSV genome, they gave similar results for 218 (91.6%) nasal aspirates. Compared with conventional methods, the sensitivity, specificity, and agreement were 97.5, 63.9, and 75.2%, respectively, for RT-PCR-1 and 89.7, 71.9, and 77.7%, respectively, for RT-PCR-2, and for these two RT-PCR assays, the positive predictive value (PPV) and the index of agreement (kappa) were comparable and moderate, respectively: PPV was 57.8%...Continue Reading
References
Apr 1, 1992·Journal of Clinical Microbiology·A W PatonR J Harris
Dec 1, 1992·Journal of Medical Virology·H A CubieB Totapally
Feb 1, 1991·The Journal of General Virology·P A Cane, C R Pringle
Mar 1, 1991·Journal of Clinical Microbiology·S TakimotoC H Martinez
Jun 1, 1989·The Journal of General Virology·P R Johnson, P L Collins
May 18, 1989·Nature·S Kwok, R Higuchi
Dec 1, 1986·Journal of Clinical Microbiology·F FreymuthJ F Duhamel
Jan 1, 1981·Clinical Pharmacology and Therapeutics·M S Kramer, A R Feinstein
Sep 1, 1994·Journal of Medical Virology·A J van MilaanE C Claas
Jun 1, 1993·Journal of Clinical Microbiology·J C HierholzerC T McEwen
Citations
Jul 12, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew K O'SheaGregory C Gray
Oct 14, 2004·Annals of Tropical Paediatrics·I FodhaF Freymuth
Jan 13, 2011·Revista Do Instituto De Medicina Tropical De São Paulo·Elenice StroparoSonia Mara Raboni
Apr 22, 2015·Revista paulista de pediatria : orgão oficial da Sociedade de Pediatria de São Paulo·Diane PuerariNancy Cristina Junqueira Bellei
Oct 6, 2000·Bone Marrow Transplantation·Y KishiY Muto
Oct 16, 2007·Journal of Medical Virology·Imène FodhaFrançois Freymuth
Sep 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L E LarsenA Uttenthal
Oct 4, 2003·Blood·Elias J AnaissieBart Barlogie
Nov 24, 2012·Journal of Biophotonics·Pierre Negri, Richard A Dluhy
Nov 16, 2010·Expert Review of Anti-infective Therapy·James B Mahony
Dec 22, 2011·Critical Reviews in Clinical Laboratory Sciences·James B MahonyMarek Smieja
Oct 16, 2008·Clinical Microbiology Reviews·James B Mahony
Mar 5, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Kristin C Halfpenny, David W Wright
Dec 22, 2006·Journal of Clinical Microbiology·Jennifer S Goodrich, Melissa B Miller
Jan 9, 2003·Journal of Clinical Microbiology·Aizhong HuSheau-Mei Cheng
Sep 6, 2003·Journal of Clinical Microbiology·Ann R FalseyEdward E Walsh
Mar 21, 1998·Journal of Clinical Microbiology·G Eugene-RuellanN Tordo
Apr 28, 2001·Journal of Clinical Microbiology·S C KehlJ Fan
Jul 28, 2001·Journal of Clinical Microbiology·L LioliosS Wesselingh
May 15, 1999·Journal of Clinical Microbiology·J F ValarcherF Schelcher
Mar 7, 2002·Journal of Clinical Microbiology·Ann R FalseyEdward E Walsh
Jan 11, 2000·Clinical Microbiology Reviews·W M Sullender
Jul 25, 2000·Clinical Microbiology Reviews·A R Falsey, E E Walsh
Mar 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I M Mackay
Jul 1, 1998·Seminars in Pediatric Infectious Diseases·Kelly J Henrickson
Nov 3, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Margareta Ieven
Sep 13, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B GröndahlH-J Schmitt