Detection of respiratory viruses in gargle specimens of healthy children

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Saeko MorikawaTetsuo Kase

Abstract

Respiratory tract viral infection is one of the most common and important diseases in children. Polymerase chain reaction (PCR) tests are often used to detect viruses in samples, it is difficult to interpret the clinical significance of PCR positivity, which may reflect a past, imminent or active asymptomatic infection due to their high sensitivity. Although single respiratory viruses have been detected in samples from children with symptoms, other respiratory viruses can also be detected simultaneously. However, the clinical importance of these findings for the symptoms is not known. To investigate the prevalence of respiratory viruses among children without any symptoms such as acute respiratory illness and/or fever. From week twenty-five 2013 to week twenty-six 2014, gargle samples were collected from children once a week and these samples were subjected to real-time PCR to detect respiratory viruses. On each sampling day, we asked the parents about their children's health condition. Among the 286 samples collected, 200 were from asymptomatic children. In the asymptomatic condition, human parechovirus, adenovirus, enterovirus, rhinovirus, coronavirus 229E and HKU1 were observed in 45 episodes. In samples from symptomatic chi...Continue Reading

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Citations

Nov 27, 2015·Medical Hypotheses·Patrick D Shaw Stewart
Nov 2, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Saeko MorikawaTetsuo Kase
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Methods Mentioned

BETA
PCR
for
nucleic acid amplification

Software Mentioned

MEGA5
DNADynamo

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