Detection of Rh D-associated epitopes in human and animal tissues using human monoclonal anti-D antibodies.

British Journal of Haematology
S J Thorpe

Abstract

The distribution of Rh D-associated epitopes in human and animal tissues has been assessed by immunochemical techniques using six human monoclonal anti-D antibodies. The IgM antibody MAD-2 reacted predominantly with endothelial cells lining blood vessels and with animal leucocytes and human leucocytes from both Rh D-positive and Rh D-negative donors. Immunoblotting revealed reactivity with a 55 kDa tissue component which was most prominent in homogenates of heart, lung and spleen. In contrast, the IgG antibodies UCHD4 and FOG-1 stained smooth muscle, and UCHD4 in addition weakly stained cardiac and skeletal muscle and the glomeruli of kidney. Immunoblotting with UCHD4 revealed reactivity with a 27 kDa tissue component which was most prominent in heart homogenates. The results show that the six monoclonal anti-D antibodies recognize at least four different epitopes and that Rh D-associated epitopes may occur in non-erythroid cell types.

References

Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Feb 19, 1983·Lancet·D H CrawfordE R Huehns
Dec 17, 1982·Journal of Immunological Methods·G D JohnsonE J Holborow
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S R GoodmanR R Kulikowski
Jan 1, 1962·Vox Sanguinis·P TIPPETT, R SANGER
Jul 1, 1962·Transfusion·A S WIENER, L J UNGER

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Citations

Jul 14, 1998·Immunotechnology : an International Journal of Immunological Engineering·T PatersonM C Carter
Jan 25, 2003·Scandinavian Journal of Immunology·S J ThorpeK M Thompson
Feb 1, 1993·British Journal of Haematology·S J Thorpe, S W Bailey
Jan 1, 1990·Baillière's Clinical Haematology·R Madhok, C D Forbes

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