Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay.

Journal of Clinical Microbiology
B S NiklassonT P Gargan

Abstract

A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extracted suckling mouse liver antigen. In the assay, antigen was captured by mouse antibody adsorbed to polystyrene plates and then detected by reacting sequentially with rabbit anti-Rift Valley fever virus antibody and swine anti-rabbit immunoglobulin G conjugated to alkaline phosphatase. ELISA proved to be useful in measuring viral antigen in different animal systems. However, great variation was found in the amount of antigen per PFU encountered in different circumstances. The ELISA system was optimized using supernatant fluids from infected Vero cell cultures and had a sensitivity of 10(5) PFU/ml. Hamsters develop progressive viremia, much as seen in susceptible domestic animals, such as lambs; ELISA could reliably detect 10(6) PFU/ml of viremic hamster serum. Rhesus monkeys with Rift Valley fever infection were positive by ELISA even when viremias were only 5 X 10(3) PFU/ml. ELISA also proved to be useful in measuri...Continue Reading

References

Jan 1, 1978·Journal of Biological Standardization·W J ThomasA N DeSanctis
Jan 1, 1978·Journal of Immunological Methods·M E Johansson, J A Espmark
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·J M Meegan
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·L W LaughlinR H Watten
Oct 1, 1976·The Journal of General Virology·A VollerA N Adams

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Citations

Mar 13, 2015·PLoS Neglected Tropical Diseases·A Desirée LaBeaudCharles H King
Mar 5, 2014·Transboundary and Emerging Diseases·J M FafetineL Neves
Oct 1, 1986·The Journal of Hygiene·R SwanepoelB J Barnard
Aug 23, 2011·PLoS Neglected Tropical Diseases·A Desirée LaBeaudCharles H King
Aug 6, 2008·Emerging Infectious Diseases·A Desiree LaBeaudCharles H King
Jul 8, 2017·PLoS Neglected Tropical Diseases·Elizabeth Anne Jessie CookEric Maurice Fèvre

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