Jul 14, 1988

Detection of RNAase inhibitor from different species and organs

Biochimica Et Biophysica Acta
H Bloemendal, K Jansen

Abstract

Human placental alkaline RNAase inhibitor was purified to homogeneity. Activity was measured after each purification step. The final identification of the purified protein was done by two-dimensional polyacrylamide gel electrophoresis and by immunoblotting. Antibodies were prepared by immunization of rabbits with the highly purified inhibitor. The availability of the antiserum directed against the human inhibitor enabled the detection of RNAase inhibitor from various other organs and species. This procedure has the advantage over the usual activity test in that the inhibitor can be found even if its activity has been lost.

  • References10
  • Citations2

References

  • References10
  • Citations2

Citations

Mentioned in this Paper

Immunoelectrophoresis
Spleen
SDS-PAGE
Placenta
Alkaline Ribonuclease
Antigen-Antibody Reactions
Bos indicus
Hydrogen-Ion Concentration
Liver
Felis catus

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