PMID: 2993335Jul 1, 1985Paper

Detection of rotavirus-specific IgG antibodies by immunoperoxidase assay and enzyme-linked immunosorbent assay

Journal of Virological Methods
B S ZentnerI Sarov


An indirect immunoperoxidase assay (IPA) has been developed for determination of IgG antibodies to rotavirus. The technique employed as antigen, SA-11 infected MA 104 cells, which were air-dried on glass slides and acetone-fixed. In parallel, rota-specific IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA). Specific IgG antibodies to rotavirus were determined in sera of healthy children and in sera of patients suffering from gastroenteritis. A good correlation (r = 0.92) and (r = 0.98) for healthy children and patients, respectively, was found between IPA and ELISA techniques. The IPA technique is rapid and simple and positive results, because of the intensive staining, are easily read by low-power light microscope. The potential application of IPA and ELISA methods in serodiagnosis of rotavirus infections is discussed.


Aug 9, 1975·Lancet·A S BrydenT H Flewett
Jan 1, 1976·Infection·M Majer
Feb 1, 1977·Journal of Medical Microbiology·B D SchoubO W Prozesky
Jan 1, 1978·Archives of Virology·T H Flewett, G N Woode
Jun 3, 1978·Lancet·I L ChrystieJ E Banatvala
Mar 13, 1979·Medical Microbiology and Immunology·L Brade, W A Schmidt
Jun 1, 1976·Infection and Immunity·N R BlacklowD H Smith
Oct 1, 1977·The Journal of Infectious Diseases·D E BidwellA Voller
Jul 1, 1978·The Journal of General Virology·J D AlmeidaI L Chrystie
Sep 1, 1979·Journal of Virology·M K EstesE M Smith
Feb 1, 1977·Journal of Clinical Microbiology·I D GustR F Bishop
Jan 1, 1977·The American Journal of Tropical Medicine and Hygiene·J EsparzaR M de Ilja
Jan 1, 1967·Archiv für die gesamte Virusforschung·H H Malherbe, M Strickland-Cholmley
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
May 1, 1983·Infection and Immunity·G P DavidsonC P Kirubakaran
Aug 27, 1983·British Medical Journal·K GrimwoodJ M Allan
Feb 1, 1983·Journal of Clinical Microbiology·M Riepenhoff-TaltyP L Ogra
Jan 1, 1980·Journal of Reproduction and Fertility·S PaulG P Talwar
Jan 1, 1984·Canadian Journal of Botany. Journal Canadien De Botanique·T W RuftyS C Huber

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.