Detection of RTX toxin genes in gram-negative bacteria with a set of specific probes.

Applied and Environmental Microbiology
P KuhnertJ Frey

Abstract

The family of RTX (RTX representing repeats in the structural toxin) toxins is composed of several protein toxins with a characteristic nonapeptide glycine-rich repeat motif. Most of its members were shown to have cytolytic activity. By comparing the genetic relationships of the RTX toxin genes we established a set of 10 gene probes to be used for screening as-yet-unknown RTX toxin genes in bacterial species. The probes include parts of apxIA, apxIIA, and apxIIIA from Actinobacillus pleuropneumoniae, cyaA from Bordetella pertusis, frpA from Neisseria meningitidis, prtC from Erwinia chrysanthemi, hlyA and elyA from Escherichia coli, aaltA from Actinobacillus actinomycetemcomitans and lktA from Pasteurella haemolytica. A panel of pathogenic and nonpathogenic gram-negative bacteria were investigated for the presence of RTX toxin genes. The probes detected all known genes for RTX toxins. Moreover, we found potential RTX toxin genes in several pathogenic bacterial species for which no such toxins are known yet. This indicates that RTX or RTX-like toxins are widely distributed among pathogenic gram-negative bacteria. The probes generated by PCR and the hybridization method were optimized to allow broad-range screening for RTX toxin g...Continue Reading

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Citations

Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Mar 17, 2004·FEMS Microbiology Letters·Erika Mena-RojasErasmo Negrete-Abascal
Jun 10, 2010·FEMS Microbiology Reviews·Irena LinhartováPeter Sebo
Aug 4, 2009·International Journal of Systematic and Evolutionary Microbiology·Peter KuhnertJoachim Frey
Dec 26, 2001·Veterinary Microbiology·Sanjeev Kumar NarayananGeorge C Stewart
Sep 1, 2000·Microbes and Infection·M R Ackermann, K A Brogden
Feb 28, 2001·Microbes and Infection·G GerlachR Gross

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