Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients

Viruses
Christopher KandelLee W Goneau

Abstract

Widely available and easily accessible testing for COVID-19 is a cornerstone of pandemic containment strategies. Nasopharyngeal swabs (NPS) are the currently accepted standard for sample collection but are limited by their need for collection devices and sampling by trained healthcare professionals. The aim of this study was to compare the performance of saliva to NPS in an outpatient setting. This was a prospective study conducted at three centers, which compared the performance of saliva and NPS samples collected at the time of assessment center visit. Samples were tested by real-time reverse transcription polymerase chain reaction and sensitivity and overall agreement determined between saliva and NPS. Clinical data was abstracted by chart review for select study participants. Of the 432 paired samples, 46 were positive for SARS-CoV-2, with seven discordant observed between the two sample types (four individuals testing positive only by NPS and three by saliva only). The observed agreement was 98.4% (kappa coefficient 0.91) and a composite reference standard demonstrated sensitivity of 0.91 and 0.93 for saliva and NPS samples, respectively. On average, the Ct values obtained from saliva as compared to NPS were higher by 2.76...Continue Reading

References

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Citations

Jan 14, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Christopher E KandelChristie Vermeiren
Jun 27, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Laura E BurnesRobert A Kozak
Jun 22, 2021·Frontiers in Public Health·Douglas Carvalho CaixetaRobinson Sabino-Silva
Apr 29, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paige GableUNKNOWN Centers for Disease Control and Prevention COVID-19 Laboratory Task Force
Aug 6, 2021·PloS One·Jennifer R HamiltonUNKNOWN IGI SARS-CoV-2 Consortium
Sep 4, 2021·The American Journal of Emergency Medicine·Katia BruxvoortSteven J Jacobsen
Oct 8, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jeannot DumaresqAnnie-Claude Labbé

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Methods Mentioned

BETA
PCR
nucleic acid amplification

Software Mentioned

R

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