Detection of SARS-CoV-2 IgG antibodies in dried blood spots.

Diagnostic Microbiology and Infectious Disease
Coleman T TurgeonElitza S Theel

Abstract

The COVID-19 pandemic led to development of numerous serologic tests. To obviate the need for phlebotomy services, we validated dried blood spots (DBS) for anti-SARS-CoV-2 serologic testing. Immunoglobulins were extracted from 3 mm DBS punches and the extracts were analyzed using the Euroimmun anti-SARS-CoV-2 IgG ELISA. Various pre-analytical factors were studied. Results were favorable for DBS stored for at least 67 days at or below 37°C. Comparison between paired serum and DBS specimens tested by the Euroimmun ELISA showed 96.8% and 81.3% positive and negative agreement, respectively, indicating that confirmatory testing of positive Euroimmun results on DBS extracts is necessary to achieve clinical accuracy. Our findings suggest that any SARS-CoV-2 antibody assay that requires pre-dilution of serum is amenable to DBS as an alternate specimen type that is relatively low cost, self-collectable, stable, can be shipped by standard mail and could be used to establish the seroprevalence of large populations.

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May 1, 2020·Journal of Clinical Microbiology·Elitza S TheelKamran Kadkhoda
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May 8, 2021·Mayo Clinic Proceedings·Rickey E CarterUNKNOWN Mayo Clinic Serology Screening Program Operations Team

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Citations

Oct 21, 2021·Microbiology Spectrum·Hannah L ItellJulie Overbaugh

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