Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak

The Science of the Total Environment
A. HataRyo Honda

Abstract

The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater samples has been documented in several countries. Wastewater-based epidemiology (WBE) is potentially effective for early warning of a COVID-19 outbreak. In this study, presence of SARS-CoV-2 RNA in wastewater samples was investigated and was compared with the number of the confirmed COVID-19 cases in the study area during COVID-19 outbreak in Japan. In total, 45 influent wastewater samples were collected from five wastewater treatment plants in Ishikawa and Toyama prefectures in Japan. During the study period, the numbers of confirmed COVID-19 cases in these prefectures increased from 0.3 and 0 to >20 per 100,000 people. SARS-CoV-2 ribonucleic acid (RNA) in the samples was detected using several PCR-based assays. Of the 45 samples, 21 were positive for SARS-CoV-2 according to at least one of the three quantitative RT-PCR assays. The detection frequency increased when the number of total confirmed SARS-CoV-2 cases in 100,000 people exceeded 10 in each prefecture; however, SARS-CoV-2 could also be detected at a low frequency even when the number was below 1.0. SARS-CoV-2 in wastewater could be detected in the early stage of the epidemic, ev...Continue Reading

References

May 15, 2002·Journal of Virological Methods·Kirsten A MooijmanArie H Havelaar
Nov 16, 2004·Journal of Virological Methods·Matthew R OlsonRandall E Hicks
Feb 20, 2009·Journal of Fish Diseases·E HaramotoS Ohgaki
Aug 4, 2009·Applied and Environmental Microbiology·Tineke H Jones, Michael W Johns
Nov 17, 2009·Applied and Environmental Microbiology·Mie N HonjoZen'ichiro Kawabata
Apr 19, 2011·Applied and Environmental Microbiology·Nathalie DeboosereMichèle Vialette
Dec 25, 2012·Applied and Environmental Microbiology·Donna S FrancyH D Alan Lindquist
May 9, 2015·Environmental Science & Technology·Zhugen YangKevin V Thomas
Mar 25, 2019·Food and Environmental Virology·Albert BlancoAlbert Bosch
Feb 8, 2020·Clinical Chemistry·Daniel K W ChuLeo L M Poon
Feb 20, 2020·Japanese Journal of Infectious Diseases·Kazuya ShiratoMakoto Takeda
Mar 10, 2020·Emerging Infectious Diseases·An TangJian-Bo Yan
Mar 18, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hiroshi NishiuraNatalie M Linton
Mar 19, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Kenji MizumotoGerardo Chowell
Mar 23, 2020·The Lancet. Gastroenterology & Hepatology·Yongjian WuXi Huang
Apr 3, 2020·Nature·Roman WölfelClemens Wendtner
Apr 10, 2020·Journal of Clinical Microbiology·Arun K NallaAlexander L Greninger
Apr 23, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ryosuke OmoriGerardo Chowell
May 19, 2020·Environmental Science & Technology·Akihiko Hata, Ryo Honda
May 20, 2020·Water Research·Walter RandazzoGloria Sánchez
May 29, 2020·The Science of the Total Environment·Giuseppina La RosaElisabetta Suffredini
Jul 1, 2020·Nature·Enrico LavezzoUNKNOWN Imperial College COVID-19 Response Team
Jul 12, 2020·Nature Microbiology·Chantal B F VogelsNathan D Grubaugh
Jul 22, 2020·The Science of the Total Environment·Sara Giordana RimoldiFranco Salerno
Aug 8, 2020·The Science of the Total Environment·Samendra P SherchanMasaaki Kitajima
Aug 8, 2020·The Science of the Total Environment·Masaaki KitajimaJoan B Rose
Aug 14, 2020·The Science of the Total Environment·Eiji HaramotoMasaaki Kitajima
Sep 10, 2020·Cell Reports Medicine·Artem NemudryiBlake Wiedenheft

❮ Previous
Next ❯

Citations

Mar 2, 2021·Chemical Engineering Journal·Xuan LiGuangming Jiang
May 20, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·The Son LeShinya Maenosono
May 30, 2021·The Science of the Total Environment·Xuan LiGuangming Jiang
Jun 7, 2021·The Science of the Total Environment·Xiaoqing XuTong Zhang
Jun 18, 2021·The Science of the Total Environment·Vu Duc CanhHiroyuki Katayama
Jun 18, 2021·Environmental Science and Pollution Research International·Hadil M AlahdalAhmed Alahmari
Jun 29, 2021·Bioresource Technology Reports·Beenish SabaAnn D Christy
Jul 30, 2021·The Science of the Total Environment·Tim BoogaertsPeter Delputte
Jul 30, 2021·Environment International·Eusebi CalleLluís Corominas
Jul 19, 2021·The Science of the Total Environment·Maximiliano Giraud-BilloudIsrael A Vega
Jul 3, 2021·Environmental Science and Pollution Research International·Tanmay DharmadhikariMahesh Dharne
Aug 9, 2021·The Science of the Total Environment·Emma J PalmerMary Jo Kirisits
Sep 2, 2021·The Science of the Total Environment·Maria Paola TomasinoAna P Mucha
Oct 2, 2021·Scientific Reports·Naďa KrivoňákováTomáš Mackuľak
Oct 11, 2021·The Science of the Total Environment·Sara CastiglioniSandro Binda

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN908947.3
AB435515.1
MN908947

Methods Mentioned

BETA
PCR
amplicon sequencing
electrophoresis
nucleic acid extraction

Software Mentioned

R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.