Detection of second-line drug resistance in Mycobacterium tuberculosis using oligonucleotide microarrays

BMC Infectious Diseases
Danila V ZimenkovDmitry A Gryadunov

Abstract

The steady rise in the spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) requires rapid and reliable methods to identify resistant strains. The current molecular methods to detect MTB resistance to second-line drugs either do not cover an extended spectrum of mutations to be identified or are not easily implemented in clinical laboratories. A rapid molecular technique for the detection of resistance to second-line drugs in M. tuberculosis has been developed using hybridisation analysis on microarrays. The method allows the identification of mutations within the gyrA and gyrB genes responsible for fluoroquinolones resistance and mutations within the rrs gene and the eis promoter region associated with the resistance to injectable aminoglycosides and a cyclic peptide, capreomycin. The method was tested on 65 M. tuberculosis clinical isolates with different resistance spectra that were characterised by their resistance to ofloxacin, levofloxacin, moxifloxacin, kanamycin and capreomycin. Also, a total of 61 clinical specimens of various origin (e.g., sputum, bronchioalveolar lavage) were tested. The sensitivity and specificity of the method in the detection of resistance to flu...Continue Reading

References

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Citations

Mar 2, 2016·The Journal of Antimicrobial Chemotherapy·Danila V ZimenkovDmitry A Gryadunov
Mar 1, 2015·Biochimica Et Biophysica Acta·Simone Maria-NetoOctávio Luiz Franco
Jan 1, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·W-L HuangRuwen Jou
May 13, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Julia A BespyatykhVadim M Govorun
Jan 31, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Young Seok LeeTae Sun Shim
Jan 23, 2015·Journal of Clinical Microbiology·Danila V ZimenkovDmitry A Gryadunov
Apr 30, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fernanda MaruriTimothy R Sterling
Jun 24, 2018·Analytical and Bioanalytical Chemistry·Elena V KostinaVladimir A Ryabinin
Apr 25, 2015·British Journal of Biomedical Science·J A O'ConnorB Lucey
May 7, 2019·Frontiers in Microbiology·Thi Ngoc Anh NguyenThi Van Anh Nguyen

Related Concepts

Aminoglycosides
Antibiotics
Bacterial Proteins
DNA, Bacterial
Fungus Drug Sensitivity Tests
Mycobacterium tuberculosis H37Rv
Sputum, Induced
Tuberculosis, Drug-Resistant
Cdna Microarrays
Fluoroquinolones

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