May 28, 2013

Detection of second-line drug resistance in Mycobacterium tuberculosis using oligonucleotide microarrays

BMC Infectious Diseases
Danila ZimenkovD Gryadunov

Abstract

The steady rise in the spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) requires rapid and reliable methods to identify resistant strains. The current molecular methods to detect MTB resistance to second-line drugs either do not cover an extended spectrum of mutations to be identified or are not easily implemented in clinical laboratories. A rapid molecular technique for the detection of resistance to second-line drugs in M. tuberculosis has been developed using hybridisation analysis on microarrays. The method allows the identification of mutations within the gyrA and gyrB genes responsible for fluoroquinolones resistance and mutations within the rrs gene and the eis promoter region associated with the resistance to injectable aminoglycosides and a cyclic peptide, capreomycin. The method was tested on 65 M. tuberculosis clinical isolates with different resistance spectra that were characterised by their resistance to ofloxacin, levofloxacin, moxifloxacin, kanamycin and capreomycin. Also, a total of 61 clinical specimens of various origin (e.g., sputum, bronchioalveolar lavage) were tested. The sensitivity and specificity of the method in the detection of resistance to flu...Continue Reading

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  • Citations16

References

Mentioned in this Paper

Fluoroquinolone antiinfectives, ophthalmologic
Bacterial Proteins
Extensively Drug-Resistant Tuberculosis
Fluoroquinolones
Fluoroquinolone antibacterials, systemic
Moxifloxacin
Aminoglycosides
Bronchoalveolar Lavage
Sputum, Induced
Smear Sample

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