Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction

Chemical & Pharmaceutical Bulletin
Ryu MochizukiT Toida

Abstract

We developed a simple and sensitive HPLC method for the determination of selenocyanate (SeCN-). The König reaction, which is generally used for the determination of cyanide and thiocyanate, was applied for the post-column detection, and using barbituric acid as a fluorogenic reagent made it possible to detect SeCN- with high sensitivity. The limits of detection (LOD) and quantification (LOQ) were 73.5 fmol and 245.1 fmol, respectively. Subsequently, the amounts of SeCN- in human blood and in cultured cell samples were analyzed, and no SeCN- was detected in human whole blood. Interestingly, we have found that some of the spiked SeCN- decomposed to cyanide in human whole blood. Ascorbic acid suppressed the decomposition of SeCN- to cyanide by reducing the ferric ion, which is typically involved in SeCN- decomposition. Then, SeCN- was detected in cultured HEK293 cells exposed to selenite. The established HPLC method with fluorescence detection of SeCN- is useful for investigating small amounts of SeCN- in biological samples.

References

Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yayoi KobayashiKazuo T Suzuki
Oct 1, 1952·Proceedings of the Society for Experimental Biology and Medicine·K L PINES, M M CRYMBLE
Oct 2, 2004·Clinical Chemistry·Kevin A Francesconi, Florence Pannier
Aug 6, 2015·Chemical Research in Toxicology·Yasumi AnanYasumitsu Ogra

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