PMID: 10333Oct 1, 1976

Detection of serum proteins in the electrophoretic patterns of total proteins of mycoplasma cells

The Journal of Hygiene
O E Yaguzhinskaya

Abstract

The contamination of mycoplasma cell preparations by serum proteins originating from culture medium was studied. A. laidlawii and M. arthritidis cells were grown in the presence of [14C]-aminoacids, and the cells were washed with 0-9% NaC1 by threefold centrifugation. Total proteins of the washed cells were analysed by SDS gel electrophoresis. Coomassie-stained electrophoretic patterns were compared with autoradiographs of the same gels. The stained electrophoretic pattern of washed A. laidlawii grown without serum was identical with autoradiographs of the same cells grown without or with serum. That of washed A. laidlawii grown with serum differed from the corresponding autoradiography by the presence of extra protein bands I, II, III, and IV with molecular weights of over 160,000, 80,000-87,000, 55,000 and 25,000, respectively. The same extra bands were found in stained electrophoretic patterns of washed: (a) A. laidlawii cells grown without serum and mixed with serum in the stationary phase, (b) M. arthritidis cells, as compared with their autoradiographs, (c) serum precipitate. The bands III and IV may be due to the heavy and light chains of gamma-globulin, the band II might belong to transferrin or to some component of com...Continue Reading

References

Feb 1, 1970·Analytical Biochemistry·S Ward
Apr 16, 1973·Biochimica Et Biophysica Acta·S RottemS Razin
Apr 5, 1973·Journal of Molecular Biology·J King, U K Laemmli
Feb 1, 1969·Journal of Bacteriology·J D PollackL B Senterfit
Mar 1, 1971·Experimental Cell Research·G J TodaroE Rands
Dec 28, 1971·Journal of Molecular Biology·J King, U K Laemmli
Nov 1, 1969·Journal of Bacteriology·R P LipmanF W Denny
Feb 1, 1968·Journal of General Microbiology·E T CheltonR T Walker
Jan 1, 1968·Ergonomics·L Lewillie, R Sneppe

Citations

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K S WiseR T Action
Jul 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·E BertholdK Sachse
Sep 1, 1995·FEMS Immunology and Medical Microbiology·G Tangen, H Kirchhoff

Related Concepts

Acholeplasma laidlawii
Autoradiography
Bacterial Proteins
Serum Proteins
Centrifugation, Isopycnic
SDS-PAGE
Hydrogen-Ion Concentration
Mycoplasma putrefaciens
Irium

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