Detection of sialuria by cation-exchange high-performance liquid chromatography

Journal of Chromatography
I Mononen

Abstract

A simple and selective method for the detection of sialuria by high-performance liquid chromatography is described. The urine sample (2 ml) is purified using a C18 cartridge or ion-exchange chromatography, and free N-acetylneuraminic acid is separated on an Aminex HPX-87 cation-exchange column using 3 mM sulphuric acid as the mobile phase. The retention time of N-acetylneuraminic acid is ca. 8 min and the detection limit ca. 1 mumol/l. The within-day coefficient of variation is less than 4.9% and the day-to-day coefficient of variation is less than 5.6%. The method was tested on twenty normal individuals and four sialuria patients.

References

Nov 1, 1979·European Journal of Biochemistry·M RenlundS L Koskela
May 12, 1975·Analytical Biochemistry·J Casals-StenzelR Schauer
Aug 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·P P CardoR Gatti
Feb 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·J PaloK Palmgren
Jan 17, 1983·European Journal of Biochemistry·M RenlundT Krusius
Mar 28, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·J C MichalskiG Strecker

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Citations

Jun 15, 1988·Analytical Chemistry·H G BarthF E Regnier

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