Detection of single cells secreting IFN-gamma, IL-6, and IL-10 in irreversibly rejected human kidney allografts, and their modulation by IL-2 and IL-4

Transplantation
P MervilleJ Banchereau

Abstract

The frequency of isolated low-density graft-infiltrating cells (GIC) secreting IFN-gamma, IL-6, and IL-10 was studied in 8 cases of irreversible rejection of human renal allografts, using ELISA and SPOT-forming cells (ELISPOT) assays. The GIC were mostly CD8+ T cells, although CD4+ T cells, B cells, and macrophages could also be detected. On the average, in 10(6) cells, 189 secreted IFN-gamma, 747 IL-10, and 17114 IL-6. Culture of GIC in the presence of IL-2 resulted in an increase in the frequency of IFN-gamma-producing cells (IFN-gamma-PC), in a dose-dependent manner, with an optimal 6.5-fold increase at 50 U/ml. In contrast, 25 U/ml IL-4 decreased the frequency of spontaneous and IL-2-induced IFN-gamma-PC by 71.3% and 52.8%, respectively. Furthermore, IL-4 reduced the frequency of IL-6-PC (56.8%). By this new approach to graft rejection, it becomes easier to determine cytokine profiles within an allograft and to study their modulation by different agents. It could be a useful model of study for the development of new treatment.

Citations

Jan 1, 1995·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·C C Nast
Oct 1, 1993·Current Opinion in Immunology·M J Dallman
Oct 1, 1995·Current Opinion in Immunology·J S Bromberg
Jun 9, 2005·Fish & Shellfish Immunology·Uwe FischerTeruyuki Nakanishi
Feb 12, 2000·Journal of Immunological Methods·S JanetzkiA N Houghton
Dec 1, 1999·Transplantation Proceedings·N IshidoN Tanaka
Dec 24, 1997·Transplant Immunology·A M de MattosA C Bakke
Jul 4, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Joannis E VamvakopoulosSu M Metcalf
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G BlanchoC LeGuern
Jan 27, 1996·Transplantation·D L LedinghamT D Lee
Jun 1, 1994·Transplantation Proceedings·C PöhleinC Hammer
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·J Kaden, R Priesterjahn
Jun 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Arvilommi
Aug 6, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·J BienvenuJ P Revillard
Nov 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Tumelo Mashishi, Clive M Gray
Jun 6, 2000·Journal of Immunotherapy·A SchmittelC Scheibenbogen
Jan 5, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J FangmannA Bachmann
Jun 12, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Yang HengFeili Gong
Mar 16, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·Rolf WeimerGerhard Opelz
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·O Le MoineJ Devière
Oct 10, 2012·Cell Transplantation·Makiko Kumagai-BraeschAnnika Tibell
May 31, 2018·The Korean Journal of Internal Medicine·Byung Ha ChungMi-La Cho
Apr 29, 1998·Immunology and Cell Biology·K J ErbG Le Gros
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·C PöhleinC Hammer

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