Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleases

Analytical Biochemistry
Yen-Ting ChenShao-Yi Hou

Abstract

The current study reports an assay approach that can detect single-nucleotide polymorphisms (SNPs) and identify the position of the point mutation through a single-strand-specific nuclease reaction and a gold nanoparticle assembly. The assay can be implemented via three steps: a single-strand-specific nuclease reaction that allows the enzyme to truncate the mutant DNA; a purification step that uses capture probe-gold nanoparticles and centrifugation; and a hybridization reaction that induces detector probe-gold nanoparticles, capture probe-gold nanoparticles, and the target DNA to form large DNA-linked three-dimensional aggregates of gold nanoparticles. At high temperature (63 degrees C in the current case), the purple color of the perfect match solution would not change to red, whereas a mismatched solution becomes red as the assembled gold nanoparticles separate. Using melting analysis, the position of the point mutation could be identified. This assay provides a convenient colorimetric detection that enables point mutation identification without the need for expensive mass spectrometry. To our knowledge, this is the first report concerning SNP detection based on a single-strand-specific nuclease reaction and a gold nanoparti...Continue Reading

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Citations

Jul 13, 2012·Accounts of Chemical Research·Xiaoji XieXiaogang Liu
Jan 18, 2011·Chemical Communications : Chem Comm·Chao ShiCuiping Ma
Jun 20, 2013·Journal of Bioscience and Bioengineering·Ming-Shu LinShao-Yi Hou
Feb 25, 2009·Journal of Microbiological Methods·Shiuh-Bin FangShao-Yi Hou
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Oct 19, 2010·Micron : the International Research and Review Journal for Microscopy·Dung The NguyenKyo-Seon Kim
Jun 14, 2011·Nanomaterials·Pooja M TiwariShree R Singh
Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
Mar 14, 2021·Analytical and Bioanalytical Chemistry·Loan Thi NgoTze-Ta Huang

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