Detection of Sirtuin-1 protein expression in peripheral blood leukocytes in dogs

The Journal of Veterinary Medical Science
Kuniko YoshimuraYasuyuki Momoi

Abstract

Sirtuin-1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase with a large number of protein substrates. It has attracted a lot of attention in association with extending lifespan. The objective of this study was to enable the evaluation of SIRT1 expression in peripheral blood mononuclear cells (PBMCs) from dogs by flow cytometry. Three transcript variants were amplified from PBMCs by reverse transcription PCR and the nucleotide sequences were analyzed. On the basis of deduced amino acid sequence, a monoclonal antibody against human SIRT1, 1F3, was selected to detect canine SIRT1. Canine SIRT1 in peripheral blood mononuclear cells was successfully detected by western blotting using this antibody. Intracellular canine SIRT1 was also detected in permeabilized 293T cells transfected with a canine SIRT1 expression plasmid by flow cytometry using this antibody. SIRT1 was detected in all leukocyte subsets including lymphocytes, granulocytes and monocytes. The expression level was markedly different among individual dogs. These results indicated that the method applied in this study is useful for evaluating canine SIRT1 levels in PBMCs from dogs.

References

Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
Oct 24, 2001·Cell·H VaziriR A Weinberg
Jul 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·S H MADIN, N B DARBY
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Blanka Rogina, Stephen L Helfand
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Enzo NisoliEmilio Clementi
Dec 13, 2005·Science·Danica ChenLeonard Guarente
Sep 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ilwola MattagajasinghKaikobad Irani
Feb 13, 2008·The Journal of Pharmacology and Experimental Therapeutics·Kyle A Rasbach, Rick G Schnellmann
Jun 12, 2008·FEBS Letters·Yariv KanfiHaim Y Cohen
Feb 4, 2009·Molecular and Cellular Biology·Natalia PediconiMassimo Levrero
Jul 11, 2009·Science·Ricki J ColmanRichard Weindruch
Jan 19, 2010·Annual Review of Pathology·Marcia C Haigis, David A Sinclair
Mar 17, 2010·Trends in Pharmacological Sciences·Shin-ichiro Imai, Leonard Guarente
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yong-Ru ChenWen-Can Xu
Nov 4, 2010·The Biochemical Journal·Agnieszka A KendrickKaren R Jonscher
Dec 15, 2010·The Journal of Biological Chemistry·Shiqin XiongR Wayne Alexander
Aug 1, 2012·PloS One·Hassina MassudiGilles J Guillemin
Oct 30, 2012·Free Radical Biology & Medicine·Brian J Morris
Feb 7, 2013·Physical Review Letters·M AblikimUNKNOWN BESIII Collaboration
Oct 1, 2013·BMC Veterinary Research·Shingo IshikawaToshiro Arai
Oct 30, 2013·FEMS Yeast Research·Margaret B Wierman, Jeffrey S Smith
Jan 21, 2014·Trends in Pharmacological Sciences·Basil P Hubbard, David A Sinclair
Jun 5, 2015·Respirology : Official Journal of the Asian Pacific Society of Respirology·Yajun WangBin Wu
Sep 5, 2015·Obesity Surgery·Stefania MarianiLucio Gnessi
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·K H PietiläinenM Ollikainen
Nov 18, 2015·The Journal of Clinical Endocrinology and Metabolism·Sakari JukarainenKirsi H Pietiläinen

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Methods Mentioned

BETA
PCR
PCRs
protein assay
flow cytometry
Transfection
glycosylation
enzyme linked immunosorbent assay
ELISA

Software Mentioned

FACSCalibur
CellQuest
ATGpr
AceView

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