Detection of soluble urokinase receptor by immunoradiometric assay and its application in tumor patients

Thrombosis Research
W GaoC Ruan

Abstract

It has recently been found that tumor cells express large amounts of urokinase receptor (uPAR) on their surface and that the blood soluble uPAR (suPAR) level in cancer patients is increased. However, the significance of suPAR in tumor progression is still unclear. To investigate the significance of suPAR in evaluating clinical status of solid tumor patients, an immunoradiometric assay (IRMA) based on using two monoclonal antibodies (McAbs) to different epitopes of uPAR was established to determine the serum levels of suPAR in normal individuals and solid tumor patients. The detectable range of this suPAR IRMA was 1.95-500 microg/l. The affinity constant was 4.75x10(9) l/mol. The mean rate of recovery was 101.3%, and the mean coefficients of variation for intra- and interassay were 6.40+/-2.57% (mean+/-S.D., n = 11) and 10.48+/-2.65% (n = 5), respectively. The serum suPAR levels were 2.71+/-1.12 microg/l in 62 normal individuals, 3.71+/-1.69 microg/l in 30 patients with benign tumors, and 5.82+/-2.27 microg/l in 124 patients with malignant tumors. The serum suPAR levels of these two types of tumor patients were increased in comparison with that of normal individuals (P values less than.01 and.001). The extent of their increase i...Continue Reading

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Citations

Oct 18, 2003·Clinical Biochemistry·Randa Abdel-Wahab Reda Mabrouk, Randa Ali-Labib
Nov 10, 2012·Journal of the American Society of Nephrology : JASN·Changli WeiUNKNOWN PodoNet and FSGS CT Study Consortia
Apr 29, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P WittenhagenJ Eugen-Olsen
Jan 14, 2017·Critical Reviews in Clinical Laboratory Sciences·S DesmedtM M Speeckaert
Nov 5, 2003·Medicinal Research Reviews·Charles Edo de Bock, Yao Wang

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