Abstract
It has recently been found that tumor cells express large amounts of urokinase receptor (uPAR) on their surface and that the blood soluble uPAR (suPAR) level in cancer patients is increased. However, the significance of suPAR in tumor progression is still unclear. To investigate the significance of suPAR in evaluating clinical status of solid tumor patients, an immunoradiometric assay (IRMA) based on using two monoclonal antibodies (McAbs) to different epitopes of uPAR was established to determine the serum levels of suPAR in normal individuals and solid tumor patients. The detectable range of this suPAR IRMA was 1.95-500 microg/l. The affinity constant was 4.75x10(9) l/mol. The mean rate of recovery was 101.3%, and the mean coefficients of variation for intra- and interassay were 6.40+/-2.57% (mean+/-S.D., n = 11) and 10.48+/-2.65% (n = 5), respectively. The serum suPAR levels were 2.71+/-1.12 microg/l in 62 normal individuals, 3.71+/-1.69 microg/l in 30 patients with benign tumors, and 5.82+/-2.27 microg/l in 124 patients with malignant tumors. The serum suPAR levels of these two types of tumor patients were increased in comparison with that of normal individuals (P values less than.01 and.001). The extent of their increase i...Continue Reading
References
Sep 1, 1992·European Journal of Biochemistry·L B MøllerF Blasi
Oct 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·U Reuning, N U Bang
Jul 1, 1991·Seminars in Thrombosis and Hemostasis·J C Kirchheimer, B R Binder
Dec 1, 1994·American Journal of Clinical Pathology·T PlesnerK Danø
Apr 1, 1994·Clinical Immunology and Immunopathology·I F MizukamiR F Todd
May 15, 1994·International Journal of Cancer. Journal International Du Cancer·J RømerN Brünner
Apr 15, 1996·The Journal of Clinical Investigation·M R GyetkoG B Toews
Feb 21, 1997·The Journal of Biological Chemistry·A A HigaziD B Cines
Apr 1, 1997·International Journal of Hematology·H NinomiyaT Abe
Jul 1, 1997·The Journal of Clinical Investigation·D A WaltzH A Chapman
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·H De WitteT Benraad
Aug 7, 1998·Breast Cancer Research and Treatment·H C ChungJ J Kim
Sep 19, 1998·Cell·K K WaryF G Giancotti
Oct 9, 1998·The Journal of Biological Chemistry·P FrancoM P Stoppelli
Jun 5, 1999·Seminars in Thrombosis and Hemostasis·V A Carroll, B R Binder
Jul 27, 1999·The Biochemical Journal·D A FishmanM S Stack
Sep 25, 1999·British Journal of Cancer·C Holst-HansenR W Stephens
Dec 14, 1999·Cancer·J AbeS Nakamura
Citations
Jun 4, 2002·International Journal of Hematology·Weiqiang GaoChanggeng Ruan
Oct 18, 2003·Clinical Biochemistry·Randa Abdel-Wahab Reda Mabrouk, Randa Ali-Labib
Oct 28, 2008·Pancreas·Vishnu C Ramani, Randy S Haun
Nov 10, 2012·Journal of the American Society of Nephrology : JASN·Changli WeiUNKNOWN PodoNet and FSGS CT Study Consortia
Apr 29, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P WittenhagenJ Eugen-Olsen
Jan 14, 2017·Critical Reviews in Clinical Laboratory Sciences·S DesmedtM M Speeckaert
Nov 5, 2003·Medicinal Research Reviews·Charles Edo de Bock, Yao Wang
Jun 13, 2018·Journal of Cancer Research and Therapeutics·Turkan Ozturk TopcuFazil Aydin