PMID: 7545852Apr 1, 1995Paper

Detection of specific T cell reactivity in sheep infected with Mycobacterium avium subspecies silvaticum and paratuberculosis using two defined mycobacterial antigens

Veterinary Immunology and Immunopathology
C BurrellsJ M Sharp

Abstract

A 30 kDa antigen (P30) from Mycobacterium avium ssp. paratuberculosis (M. a. paratuberculosis) and a 40 kDa (P40) antigen from Mycobacterium avium ssp. silvaticum (M. a. silvaticum) were employed in two different assays to measure the cell-mediated immune reactivity of ovine peripheral blood lymphocytes. In lymphocyte stimulation assays, proliferative responses to the P30 were observed only with lymphocytes from sheep inoculated with live M. a. paratuberculosis or M. a. silvaticum. Although this antigen was not subspecies-specific it differentiated between animals given live organisms and those inoculated with an inactive lysate. The P40 protein from M. a. silvaticum showed subspecies specificity by eliciting in vitro responses only with lymphocytes derived from sheep inoculated with live M. a. silvaticum. Similar results were obtained using an interferon-gamma release assay which proved to be a more rapid and sensitive system.

References

May 1, 1991·Research in Veterinary Science·V RitaccoA Nader
May 1, 1990·Veterinary Immunology and Immunopathology·P R WoodS L Jones

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Citations

Apr 1, 1997·Journal of Comparative Pathology·C J Clarke
May 18, 2001·Veterinary Immunology and Immunopathology·J R Stabel, R H Whitlock
Jul 10, 1998·Veterinary Immunology and Immunopathology·I Begara-McGorumD G Jones
Mar 29, 2002·The Veterinary Quarterly·I OlsenB Djønne
Jan 5, 2002·Antisense & Nucleic Acid Drug Development·R RankinS van Drunen Littel-van den Hurk
Apr 13, 2011·Veterinary Microbiology·Heidi MikkelsenGregers Jungersen
Apr 12, 2008·Veterinary Immunology and Immunopathology·Deborah L TaylorRichard J Whittington
May 1, 2007·Veterinary Microbiology·Murray E HinesRobert H Whitlock
Oct 30, 2016·The Veterinary Quarterly·Kundan Kumar ChaubeyAbdollah Derakhshandeh
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones

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