Detection of Specific ZIKV IgM in Travelers Using a Multiplexed Flavivirus Microsphere Immunoassay

Viruses
Carmel T TaylorFrederick A Moore

Abstract

Zika virus (ZIKV) has spread widely in the Pacific and recently throughout the Americas. Unless detected by RT-PCR, confirming an acute ZIKV infection can be challenging. We developed and validated a multiplexed flavivirus immunoglobulin M (IgM) microsphere immunoassay (flaviMIA) which can differentiate ZIKV-specific IgM from that due to other flavivirus infections in humans. The flaviMIA bound 12 inactivated flavivirus antigens, including those from ZIKV and yellow fever virus (YFV), to distinct anti-flavivirus antibody coupled beads. These beads were used to interrogate sera from patients with suspected ZIKV infection following travel to relevant countries. FlaviMIA results were validated by comparison to the ZIKV plaque reduction neutralization test (PRNT). The results highlight the complexity of serological ZIKV diagnosis, particularly in patients previously exposed to or vaccinated against other flaviviruses. We confirmed 99 patients with ZIKV infection by a combination of RT-PCR and serology. Importantly, ZIKV antibodies could be discriminated from those ascribed to other flavivirus infections. Serological results were sometimes confounded by the presence of pre-existing antibodies attributed to previous flavivirus infect...Continue Reading

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Citations

Jul 4, 2019·Human Vaccines & Immunotherapeutics·Christine C Roberts
Dec 13, 2019·Science Translational Medicine·Jody Hobson-PetersRoy A Hall
May 20, 2020·Diagnostics·Didier Musso, Philippe Despres
Oct 3, 2018·Tropical Medicine and Infectious Disease·Alyssa T PykeFiona May
Aug 23, 2020·Tropical Medicine and Infectious Disease·Alyssa T PykeSarah Wheatley
Mar 17, 2019·Viruses·Luis Martinez-Sobrido, Fernando Almazán
Feb 10, 2021·Medicinal Research Reviews·Xianlong ZhangTony D James

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Methods Mentioned

BETA
ELISA
ELISAs

Software Mentioned

flaviMIA
Plex Manager
Bio
Excel

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