Detection of SPG20 gene promoter-methylated DNA, as a novel epigenetic biomarker, in plasma for colorectal cancer diagnosis using the MethyLight method

Oncology Letters
Nayebali RezvaniMassoud Saidijam

Abstract

Aberrant promoter methylation of genes is a common epigenetic alteration in colorectal cancer (CRC). In the present study, spastic paraplegia 20 (SPG20) promoter-methylated DNA, as a potential diagnostic biomarker, was investigated in plasma and tumor tissue samples from patients with CRC. To the best of our knowledge, the quantification of SPG20 promoter-methylated DNA in plasma samples remains unreported. SPG20 promoter methylation was investigated in 32 paired tumor and healthy adjacent tissues, 37 plasma samples from patients with CRC, and in 37 plasma samples from a healthy control group, using the MethyLight method. The percentage of methylated reference (PMR) values was determined for each sample, and the sensitivity and specificity of this unique biomarker were evaluated. PMR values were significantly higher in plasma samples from patients with CRC compared with in those from the control group (P<0.05). Plasma specimens from patients and healthy controls exhibited median PMR values of 7.7 (95% CI, 4.15-15.28) and 0.59 (95% CI, 0.14-1.12), respectively. Notably, the median PMR values were identified as 42.39 (95% CI, 27.69-72.26) and 3.61 (95% CI, 1.07-5.29) in tumor and adjacent healthy tissues, respectively. Using rece...Continue Reading

References

Aug 11, 1994·Nucleic Acids Research·S J ClarkM Frommer
Mar 29, 2000·Nucleic Acids Research·C A EadsP W Laird
May 10, 2002·The American Journal of Pathology·Annie On-On ChanAsif Rashid
Nov 13, 2004·Cancer Biology & Therapy·Kesmic JacksonMelanie Ehrlich
Sep 27, 2005·The American Journal of Gastroenterology·Wai K LeungJoseph J Y Sung
Dec 6, 2005·Nucleic Acids Research·Daniel J WeisenbergerPeter W Laird
Feb 2, 2006·CA: a Cancer Journal for Clinicians·Robert A SmithHarmon J Eyre
Mar 16, 2006·European Journal of Gastroenterology & Hepatology·Denis HeresbachBernard Branger
Mar 3, 2007·Molecular Biology of the Cell·Joanna C BakowskaCraig Blackstone
Feb 10, 2009·Cancer Science·Keishi Yamashita, Masahiko Watanabe
Mar 25, 2009·The Journal of Cell Biology·Scott W EastmanPaul D Bieniasz
May 2, 2009·Clinical Chemistry·Theo deVosCatherine Lofton-Day
Sep 24, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bo Bin LeeDuk-Hwan Kim
Apr 8, 2010·FEBS Letters·Antonia P Sagona, Harald Stenmark
Feb 18, 2011·The American Journal of Gastroenterology·Andreas HerbstFrank T Kolligs
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Jul 13, 2011·Nature Reviews. Genetics·Vardhman K RakyanStephan Beck
Oct 20, 2011·Nature Reviews. Gastroenterology & Hepatology·Victoria Valinluck Lao, William M Grady
Nov 3, 2011·Nature Reviews. Gastroenterology & Hepatology·Nikhil PawaJohn D Norton
Dec 16, 2011·BMC Medicine·Jorja D WarrenKaren A Heichman
Jul 10, 2012·Cell·Mark A Dawson, Tony Kouzarides
Feb 2, 2013·International Journal of Medical Sciences·Hao ZhangCheng-Xue Dang
Dec 24, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Nicole BethgeGuro E Lind
Sep 26, 2014·World Journal of Gastroenterology : WJG·Vittoria StiglianoMarcello Anti
Dec 4, 2014·PloS One·Keishi YamashitaMasahiko Watanabe
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 24, 2016·Diagnostic Pathology·Luca Reggiani BonettiCristian Palmiere

❮ Previous
Next ❯

Citations

Dec 14, 2018·Experimental and Therapeutic Medicine·Ranran PanShiwei Duan
Jun 13, 2020·Frontiers in Bioengineering and Biotechnology·Shiqi ZhangYu-Dong Cai
Oct 9, 2020·International Journal of Colorectal Disease·Eivor Alette LaugsandFrank Skorpen
Jan 28, 2021·International Journal of Molecular Sciences·Luca BedonMaurizio Polano
Apr 23, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Iman AkhlaghipourMeysam Moghbeli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.