PMID: 9542934May 23, 1998Paper

Detection of staphylococcal superantigenic toxins by a CD69-specific cytofluorimetric assay measuring T-cell activation

Journal of Clinical Microbiology
Gerard LinaJerome Etienne

Abstract

The presence of staphylococcal superantigenic toxins in the supernatants of liquid cultures was detected by an easy and rapid method assessing the activation of T lymphocytes by cytofluorimetric measurement of CD69 expression. Staphylococcus aureus cells were grown in Eagle's minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. aureus strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, and exfoliative toxins A and B, induced CD69 expression in a significantly higher number of T cells than a cutoff of 2%. This CD69 assay might be used for initial detection of superantigens from S. aureus strains isolated in the context of staphylococcal toxemia or related chronic human diseases such as atopic dermatitis or Kawasaki syndrome.

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Citations

Mar 4, 2009·Infection and Immunity·Damien ThomasGerard Lina
Oct 13, 2006·Current Opinion in Infectious Diseases·A D SawitzkeB C Cole
Apr 13, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F L NowrouzianE Josefsson
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G P LennonR A Kay
Feb 22, 2017·Toxins·Thunchanok RukkawattanakulNitaya Indrawattana
May 14, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M MempelD Abeck

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