Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells

Veterinary Microbiology
Elizabet A L PereyraBibiana E Dallard

Abstract

Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the behavior of these strains in contact with bovine mammary epithelial cells (MAC-T) and the expression of those genes during bacterial-cell early interactions. The genetic diversity of 20 S. aureus strains that were isolated from milk samples taken from cows with persistent-P and non-persistent-NP IMI was high, discriminated into 13 fingerprint groups. The occurrence of genes coding for S. aureus surface proteins (clfA, clfB, fnbA, fnbB, fib, cna) and biofilm formation (icaA, icaD, icaC, bap) and in vitro biofilm-forming ability was not related to strain clinical origin (NP or P). Internalization of S. aureus into MAC-T cells was strain-dependent and internalized bacteria overexpressed adherence and biofilm-forming genes compared with those that remained in the supernatant of co-cultur...Continue Reading

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Citations

Oct 6, 2020·Journal of Veterinary Science·Jaquelina J Guzmán-RodríguezAbner J Gutiérrez-Chávez
Jul 29, 2017·Frontiers in Cellular and Infection Microbiology·Muhammad ShahidBo Han
Aug 4, 2021·Environmental Microbiology Reports·Vineeth RajanTill T Bachmann
May 5, 2021·Canadian Journal of Microbiology·Isabela Schneid KroningWladimir Padilha da Silva
Nov 21, 2021·Journal of Applied Microbiology·Melina V MolivaElina B Reinoso

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