Detection of Sub-fM DNA with Target Recycling and Self-Assembly Amplification on Graphene Field-Effect Biosensors

Nano Letters
Zhaoli GaoA T Charlie Johnson

Abstract

All-electronic DNA biosensors based on graphene field-effect transistors (GFETs) offer the prospect of simple and cost-effective diagnostics. For GFET sensors based on complementary probe DNA, the sensitivity is limited by the binding affinity of the target oligonucleotide, in the nM range for 20 mer targets. We report a ∼20 000× improvement in sensitivity through the use of engineered hairpin probe DNA that allows for target recycling and hybridization chain reaction. This enables detection of 21 mer target DNA at sub-fM concentration and provides superior specificity against single-base mismatched oligomers. The work is based on a scalable fabrication process for biosensor arrays that is suitable for multiplexed detection. This approach overcomes the binding-affinity-dependent sensitivity of nucleic acid biosensors and offers a pathway toward multiplexed and label-free nucleic acid testing with high accuracy and selectivity.

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Citations

Feb 6, 2019·Biomaterials Science·André F GirãoPaula A A P Marques
Nov 5, 2019·Mikrochimica Acta·Leila SyedmoradiKobra Omidfar
Sep 25, 2019·Toxins·Nikita NekrasovIvan Bobrinetskiy
Feb 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cao-An Vu, Wen-Yih Chen
Dec 20, 2020·Biosensors·Pedro Rodrigues de AlmeidaElmo Salomão Alves
Dec 29, 2020·ACS Applied Materials & Interfaces·Sanghwan ParkChang Young Lee
Mar 8, 2019·ACS Sensors·Alireza Abi, Afsaneh Safavi
Oct 9, 2021·Journal of the American Chemical Society·Derong KongDacheng Wei
Aug 24, 2021·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Sheida Bagherzadeh-Nobari, Reza Kalantarinejad
Nov 7, 2021·Advanced Materials·Lei HuangJiangping Tu

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Methods Mentioned

BETA
biosensors
biosensor
chemical treatment
PBASE
electrophoresis
chip
chips
Budgetsensors
AFM

Software Mentioned

MATLAB

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