Detection of subgenomic mRNA of feline coronavirus by real-time polymerase chain reaction based on primer-probe energy transfer (P-sg-QPCR).

Journal of Virological Methods
Akos HornyákSándor Belák

Abstract

Feline infectious peritonitis is one of the most severe devastating diseases of the Felidae. Upon the appearance of clinical signs, a cure for the infected animal is impossible. Therefore rapid and proper diagnosis for both the presence of the causative agent, feline coronavirus (FCoV) and the manifestation of feline infectious peritonitis is of paramount importance. In the present work, a novel real-time RT-PCR method is described which is able to detect FCoV and to determine simultaneously the quantity of the viral RNA. The new assay combines the M gene subgenomic messenger RNA (sg-mRNA) detection and the quantitation of the genome copies of FCoV. In order to detect the broadest spectrum of potential FCoV variants and to achieve the most accurate results in the detection ability the new assay is applying the primer-probe energy transfer (PriProET) principle. This technology was chosen since PriProET is very robust to tolerate the nucleotide substitutions in the target area. Therefore, this technology provides a very broad-range system, which is able to detect simultaneously many variants of the virus(es) even if the target genomic regions show large scale of variations. The detection specificity of the new assay was proven by...Continue Reading

References

Jun 1, 1992·The Journal of Veterinary Medical Science·T HohdatsuH Koyama
Apr 1, 1967·The Journal of General Virology·J D Almeida, D A Tyrrell
Apr 4, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M A KennedyL N Potgieter
Sep 19, 2003·The Journal of General Virology·D D AddieO Jarrett
Dec 23, 2004·Journal of Virology·Jolanda D F de Groot-MijnesRaoul J de Groot
Jan 25, 2005·Journal of Virological Methods·Fermin A SimonsHerman F Egberink
Oct 8, 2005·Clinical and Diagnostic Laboratory Immunology·Maya KummrowHans Lutz
May 5, 2007·Journal of Feline Medicine and Surgery·Kezban Can-SahnaTuba Ciğdem Oğuzoğlu
Feb 5, 2008·Journal of Feline Medicine and Surgery·Charlotte DyeStuart G Siddell

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Citations

Apr 19, 2015·BMC Veterinary Research·Yoshikazu TanakaTomohiro Yamaguchi
May 27, 2014·The Veterinary Journal·Niels C Pedersen
Feb 27, 2014·Veterinary Pathology·A Kipar, M L Meli
Nov 17, 2019·Viruses·Sandra Felten, Katrin Hartmann
Oct 15, 2019·Frontiers in Microbiology·Yashpal Singh MalikRaj Kumar Singh
Nov 27, 2020·Journal of Clinical Microbiology·Cristina Rodríguez-GrandeDarío García de Viedma

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Methods Mentioned

BETA
FRET
PCR
FCS
electrophoresis

Software Mentioned

Oligo Analyzer
PriProET
CLUSTAL X

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