Detection of substances with alcoholic or phenolic hydroxyl groups by generation of hydrogen peroxide with imidazole and peroxyoxalate chemiluminescence

Journal of Bioluminescence and Chemiluminescence
Osamu NozakiY Kato

Abstract

On-line detection of substances with an alcoholic or phenolic hydroxyl group using imidazole and peroxyoxalate chemiluminescence was investigated qualitatively using a flow-injection method. The substances tested included six polyphenols, five monophenols and six sugars. After incubation at 80 degrees C with an imidazole buffer (pH 9.5) the substances were detected by peroxyoxalate chemiluminescence. The polyphenols tested (e.g., pyrogallol, purpurogallin, and dopamine) showed the strongest light emission. The sugars with hydroxyl groups (e.g., fructose and lactose) and the monophenols (e.g., phenol, serotonin, and beta-estradiol) produced only a weak light emission. Imidazole served two roles, it catalysed the reaction with the hydroxyl compound and initiated peroxyoxalate chemiluminescence on-line. A novel reactor formed by packing glass beads into a flow cell (Teflon) of a chemiluminometer improved the sensitivity of light detection.

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Citations

Feb 17, 2001·Luminescence : the Journal of Biological and Chemical Luminescence·P FletcherP J Worsfold
Jul 29, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·O NozakiY Kato
Jun 22, 2000·Luminescence : the Journal of Biological and Chemical Luminescence·O Nozaki, H Kawamoto
Jun 27, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuhiko TsukagoshiRiichiro Nakajima
Dec 22, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·O NozakiH Moriyama
Jan 5, 2002·Journal of Chromatography. a·O Nozaki

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