Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene

Experimental & Applied Acarology
Jitka StaraJan Hubert

Abstract

Varroa destructor is the major cause of honey bee (Apis mellifera) colony losses. Mite control is limited to several miticides. The overuse of tau-fluvalinate has resulted in resistance via a knockdown resistance (kdr) mutation in the sodium channel gene NaVChs (L925V/I/M). In this study, we used the discriminating concentration of tau-fluvalinate (0.25 µg/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (NaVCh), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites. Resistance values corresponding to the densitometry of the resistant allele were related to mite survival. In the vial test, the survival of the control group was significantly higher (70.4%) than that of the tau-fluvalinate-treated group (34.3%). Mite survival in the vial test was significantly correlated with the mean proportion of resistance values. Individuals that died after tau-fluvalinate application exhibited an average resistance value of 0.0783, whereas individuals that survived exhibited an average resistance of 0.400. The concentration ...Continue Reading

References

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Citations

Apr 16, 2019·Pest Management Science·Tomas ErbanJan Hubert
Jun 17, 2020·Emerging Topics in Life Sciences·Amélie NoëlFanny Mondet
Sep 17, 2020·Parasitology Research·Mariano HigesJoel González-Cabrera
May 14, 2021·Experimental & Applied Acarology·Nafiye KoçThomas Van Leeuwen
Sep 8, 2021·Scientific Reports·Ivelina Ivanova, Kaspar Bienefeld

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Methods Mentioned

BETA
PCR

Software Mentioned

mgcv
Clarity
GeneTools

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