Detection of tenascin-C in the nervous system of the tenascin-C mutant mouse

Journal of Neuroscience Research
N Mitrovic, M Schachner

Abstract

We have investigated the expression of tenascin-C (TN-C) in the somatosensory cortex of early postnatal mutant mice in which lacZ was reported to be expressed in place of tenascin (Saga et al.: Genes Dev 6:1821-1831, 1992). At both the mRNA and protein levels, TN-C was detected at levels lower in the mutant than in wild type animals by in situ hybridization and by immunocytochemistry using several poly- and monoclonal antibodies directed against mouse TN-C. The distribution of TN-C immunoreactivity in coronal sections was abnormal in that the barrel field boundaries in layer 4 of the somatosensory cortex could not be detected intracellularly in most cell bodies, including possibly also neurons. Western blot analysis of homogenates of brain tissue from early postnatal animals showed an abnormal pattern of protein bands immunoreactive for TN-C in mutant animals while beta-galactosidase migrated at its expected molecular weight without incorporation into fusion proteins with TN-C, suggesting disturbed splicing mechanisms. No gross disturbances in the patterning of barrel fields could be detected in the mutant mice as shown by Nissl staining. Our observations show that the mutant mouse designed to be nully disrupted for TN-C expres...Continue Reading

References

Oct 1, 1992·Genes & Development·Y SagaS Aizawa
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S JhaveriK Crossin
Apr 1, 1994·The Journal of Cell Biology·K MatsumotoR Chiquet-Ehrismann
Jul 1, 1993·Journal of Neuroscience Research·J TaylorM Schachner
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·T BabaK Ito
Jan 1, 1992·The European Journal of Neuroscience·Udo BartschMelitta Schachner

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Citations

Jan 1, 1997·Journal of Neuroscience Research·D L SettlesH P Erickson
Apr 1, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·A TuoriH Uusitalo
Oct 6, 1999·The European Journal of Neuroscience·B W KiernanC ffrench-Constant
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Martin FlückMarie-Noëlle Giraud
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·E ForsbergR Fässler
May 30, 2006·Molecular and Cellular Neurosciences·Antoine de ChevignyPierre-Marie Lledo
Dec 18, 1998·The Journal of Investigative Dermatology·Y KoyamaM Kusakabe
Apr 5, 2012·International Journal of Dermatology·Rohini C MathiasMary Augustine
May 28, 2017·Behavioural Brain Research·V StamenkovicP Andjus
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F S Jones, P L Jones
Dec 26, 2001·Journal of Neuroscience Research·C Cifuentes-DiazD Angaut-Petit

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