Detection of the bacteriocin propionicin PLG-1 with polyvalent anti-PLG-1 antiserum

Applied and Environmental Microbiology
J A Leversee, B A Glatz

Abstract

Polyclonal antibodies against the bacteriocin propionicin PLG-1 were produced in rabbits at high titer (256,000 to 512,000, as determined by indirect enzyme-linked immunosorbent assay [ELISA]). Anti-PLG-1 antiserum neutralized the antimicrobial activity of PLG-1 preparations in a well diffusion assay. Cross-reacting protein was detected using an indirect ELISA of the culture supernatant from a fed-batch fermentation of the producer strain Propionibacterium thoenii P127 within the first 24 h of incubation, but bacteriocin activity was not detected in the same culture until 217 h of incubation. Culture supernatants from 156 strains of classical dairy propionibacteria were tested by indirect ELISA at 5 and 12 days of incubation for production of cross-reacting protein and by well diffusion assay for bacteriocin activity. Cross-reacting protein was detected in 52 strains: all of the tested strains of P. thoenii, most of the strains of Propionibacterium jensenii, and a minority of the Propionibacterium acidipropionici and Propionibacterium freudenreichii strains. Of these 52 strains, only 4 strains of P. thoenii showed bacteriocin activity in a well diffusion assay. Eight bacteriocin-negative mutants of strain P127 were negative in ...Continue Reading

References

Jan 1, 1992·Applied and Environmental Microbiology·D A Grinstead, S F Barefoot
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Apr 1, 1993·Journal of Food Protection·Catherine G Nettles, Susan F Barefoot

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Citations

Feb 17, 2015·The Journal of Dairy Research·Sameh E Mohamed, Mahmoud K Tahoun
Jun 30, 2019·Archives of Oral Biology·Junko ObataTakahiko Oho
Apr 15, 2004·Journal of Agricultural and Food Chemistry·Jorge GutiérrezPablo E Hernández

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