Detection of the carbapenemase GIM-1 in Enterobacter cloacae in Germany

The Journal of Antimicrobial Chemotherapy
Axel HamprechtP Nordmann

Abstract

To characterize the mechanisms involved in reduced susceptibility to carbapenems in two Enterobacter cloacae clinical isolates. Two E. cloacae isolates recovered from different regions in Germany and showing reduced susceptibility to carbapenems were analysed. Susceptibility testing, conjugation, transformation assays, plasmid analysis, sequencing and molecular typing using rep-PCR were performed. The two clinical isolates carried the bla(GIM-1) gene and showed resistance to ertapenem, with variable MIC values of imipenem and meropenem. The isolates were clonally unrelated. The bla(GIM-1) gene was located on self-transferable and non-typeable plasmids. Both isolates harboured distinct plasmids and integron structures containing the bla(GIM-1) gene cassette. Interestingly, one of the two plasmids was able to replicate in Pseudomonas aeruginosa, demonstrating its broad host range. This is the first identification in E. cloacae of the bla(GIM-1) gene, which is responsible for reduced susceptibility to carbapenems. We showed that this gene, previously identified in P. aeruginosa, was located in a different genetic background in E. cloacae. The bla(GIM-1) gene might spread quite efficiently in Enterobacteriaceae and P. aeruginosa, a...Continue Reading

References

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Citations

Aug 21, 2013·The Journal of Antimicrobial Chemotherapy·Martin KaaseSören Gatermann
Jun 17, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P Nordmann, L Poirel
Oct 22, 2014·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Mathias HerrmannBarbara C Gärtner
Jun 5, 2015·Frontiers in Microbiology·Anne Davin-Regli, Jean-Marie Pagès
Apr 20, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Heime RieberYvonne Pfeifer
Jun 25, 2013·Diagnostic Microbiology and Infectious Disease·G CuzonP Nordmann
Oct 5, 2013·Diagnostic Microbiology and Infectious Disease·Agnese LupoAndrea Endimiani
May 3, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ling-Fu HuangChin-Lu Chang
Jan 23, 2017·Journal of Global Antimicrobial Resistance·Jing YuZiyong Sun
Dec 31, 2014·Antimicrobial Agents and Chemotherapy·Andreas F Wendel, Colin R MacKenzie
Apr 10, 2016·Applied and Environmental Microbiology·Andreas F WendelColin R MacKenzie
Dec 9, 2015·Antimicrobial Agents and Chemotherapy·Susann SkagsethHanna-Kirsti S Leiros
Jul 19, 2019·Clinical Microbiology Reviews·Anne Davin-RegliJean-Marie Pagès
Dec 14, 2016·Antimicrobial Agents and Chemotherapy·Andreas F WendelMatthias Willmann
Jun 10, 2019·Environmental Monitoring and Assessment·Veronica Fernandes, Kalisa Bogati
May 12, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Andreas F WendelColin R MacKenzie
Jan 7, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Stephan GöttigAxel Hamprecht
Nov 10, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Maria Meier, Axel Hamprecht

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