Detection of the early stage of recombinational DNA repair by silicon nanowire transistors

Nano Letters
Marco ChiesaR Garcia

Abstract

A silicon nanowire-based biosensor has been designed and applied for label-free and ultrasensitive detection of the early stage of recombinational DNA repair by RecA protein. Silicon nanowires transistors were fabricated by atomic force microscopy nanolithography and integrated into a microfluidic environment. The sensor operates by measuring the changes in the resistance of the nanowire as the biomolecular reactions proceed. We show that the nanoelectronic sensor can detect and differentiate several steps in the binding of RecA to a single-stranded DNA filament taking place on the nanowire-aqueous interface. We report relative changes in the resistance of 3.5% which are related to the interaction of 250 RecA·single-stranded DNA complexes. Spectroscopy data confirm the presence of the protein-DNA complexes on the functionalized silicon surfaces.

References

Nov 1, 1994·Journal of Biochemistry·R WatanabeS Kuramitsu
Jul 3, 2003·Journal of Bacteriology·S DattaM Vijayan
Jun 1, 2004·Protein Science : a Publication of the Protein Society·Savvas N SavvidesGabriel Waksman
Sep 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fernando PatolskyCharles M Lieber
Feb 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wayne U WangCharles M Lieber
Sep 20, 2005·Nature Biotechnology·Gengfeng ZhengCharles M Lieber
Sep 22, 2006·Nature·Roberto GallettoStephen C Kowalczykowski
Jan 18, 2007·Nature Reviews. Molecular Cell Biology·Michael M Cox
Mar 17, 2007·Critical Reviews in Biochemistry and Molecular Biology·Michael M Cox
Apr 13, 2007·Nano Letters·Cedric R LeãoAntônio J R da Silva
Dec 22, 2007·Nano Letters·Lixuan MuShuit-Tong Lee
Feb 16, 2008·Annual Review of Biochemistry·Joseph San FilippoHannah Klein
May 13, 2008·DNA Repair·Begoña CarrascoJuan C Alonso
May 24, 2008·Nature·Zhucheng ChenNikola P Pavletich
May 12, 2009·Nucleic Acids Research·Marijn T J van LoenhoutCees Dekker
May 21, 2010·Nanotechnology·Ramses V MartínezRicardo Garcia

❮ Previous
Next ❯

Citations

Feb 15, 2013·Nanoscale Research Letters·Matteo LorenzoniBruno Torre
Aug 6, 2014·Nature Nanotechnology·Ricardo GarciaElisa Riedo
Feb 6, 2013·DNA Repair·Juan C AlonsoKunio Takeyasu
Mar 9, 2017·Nanotechnology·Yu K Ryu, Ricardo Garcia
Aug 21, 2014·The Journal of Biological Chemistry·Tribhuwan YadavJuan C Alonso
May 2, 2014·Advanced Materials·Josef BrandtAlbena Lederer
Mar 22, 2020·Sensors·Songyue ChenJing Yang
Jun 21, 2018·Nano Letters·Xinzhao XuMatteo Palma

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.