Detection of the effects of triclosan (TCS) on the metabolism of VOCs in <i>HepG2</i> cells by <i>SPI-TOFMS</i>

Journal of Breath Research
Jiyang ZhangPing Cheng

Abstract

Volatile organic compounds (VOCs) emitted by organisms and cell metabolism have demonstrated great physiological and pathological values. At present, there is a great interest in the study of volatile metabolome to determine whether VOCs can serve as potential diagnostic biomarkers. In view of the sensitivity of VOCs to physiological changes, the aim of this study was to investigate alterations in VOC profiles in the in vitro headspace of HepG2 cells after exposure to Triclosan (TCS). Since the in vivo biological effects of TCS are clearly defined, several TCS-related VOCs may potentially be traced back to common cellular processes. In this study, HepG2 cells were cultured in TCS-containing medium for 2 h, and the emitted VOCs in the headspace of the culture flask were detected using a single photon ionization time-of-flight mass spectrometry (SPI-TOFMS) instrument. The control group and the TCS-treated group could be well separated by differential VOC profiles, which were related to the physiological states of the HepG2 cells. Compared to the control group, eleven and ten specific VOCs were identified in the 20 μM and 50 μM TCS-treated groups, respectively. Among them, five specific VOCs (m/z 62, 64, 70, 121 and 146) were comm...Continue Reading

References

Mar 21, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T MossF M Williams
May 23, 2001·Archives of Biochemistry and Biophysics·J VillalaínV Micol
Jul 2, 2003·Biosensors & Bioelectronics·Corrado Di NataleArnaldo D'Amico
Aug 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wolfram MiekischGabriele F E Noeldge-Schomburg
Mar 8, 2005·American Journal of Respiratory and Critical Care Medicine·Roberto F MachadoSerpil C Erzurum
Apr 14, 2007·Biomedical Chromatography : BMC·Bogusław BuszewskiAnton Amann
Apr 19, 2007·Environmental Science & Technology·E Matthew FissPeter J Vikesland
Nov 26, 2008·Cancer Cell International·Wojciech FilipiakJakob Troppmair
Jun 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Albert Tangerman
Oct 1, 2009·BMC Cancer·Amel BajtarevicAnton Amann
Oct 20, 2009·International Journal of Cancer. Journal International Du Cancer·Patricia FuchsWolfram Miekisch
Jan 9, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Wojciech FilipiakJakob Troppmair
May 27, 2010·Analytical and Bioanalytical Chemistry·C BrunnerU Oeh
Sep 8, 2010·Radiation and Environmental Biophysics·C BrunnerU Oeh
Jan 26, 2011·Cancer Biomarkers : Section a of Disease Markers·Andreas SponringJakob Troppmair
Apr 5, 2011·Journal of Applied Toxicology : JAT·Andrea B Dann, Alice Hontela
Apr 7, 2011·Current Pharmaceutical Biotechnology·Jae Kwak, George Preti
Jul 21, 2011·Journal of Biochemistry·Mika Shirasu, Kazushige Touhara
Aug 14, 2012·International Journal of Nanomedicine·Haitham AmalHossam Haick
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gennady CherednichenkoIsaac N Pessah
Nov 13, 2012·Cancer Biomarkers : Section a of Disease Markers·Yishan WangKejing Ying
Jun 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jae KwakGeorge Preti
Apr 1, 2014·Journal of Breath Research·David SmithJonathan Beauchamp
May 3, 2014·Seminars in Oncology·Wael Al-ZoughbiGerald Hoefler
Oct 16, 2015·Journal of Breath Research·A BaranskaF J van Schooten
Mar 13, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liudan ZengJiamo Fu
Feb 1, 2017·Journal of Breath Research·L BlanchetF J van Schooten
Feb 7, 2017·Journal of Breath Research·M BouzaA Sanz-Medel
Apr 7, 2015·Toxicology Reports·Charmaine AjaoMirja Salkinoja-Salonen
Dec 9, 2017·Journal of Breath Research·Flavio A FranchinaJane E Hill
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Daniela RodriguesMárcia Carvalho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.