Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR.

Journal of Clinical Microbiology
J N VelásquezM Rodríguez

Abstract

Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS.

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Citations

Mar 23, 1999·The American Journal of Gastroenterology·D J KearneyJ P Cello
Jun 15, 2000·AIDS·W OelemannJ M Peralta
Apr 3, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·X PourrutF Derouin
Sep 1, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Angela LonoTan Tian Chye
Oct 12, 2010·Clinical Microbiology Reviews·J L N BarrattD Stark
Jul 28, 2020·Journal of Fungi·Alexandra Valenčáková, Monika Sučik
May 22, 2009·Journal of Gastrointestinal Cancer·Angela R LonoTan T Chye
May 21, 2011·Transactions of the Royal Society of Tropical Medicine and Hygiene·Angela LonoTan Tian Chye
Jul 10, 2019·Acta Parasitologica·Jorge Néstor VelásquezSilvana Carnevale
May 9, 2000·Clinical and Diagnostic Laboratory Immunology·S CarnevaleM I Rodríguez
Aug 7, 2009·Interdisciplinary Perspectives on Infectious Diseases·Kaya Ghosh, Louis M Weiss
Jul 24, 2015·Acta Parasitologica·Silvana CarnevaleJorge Néstor Velásquez
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Jul 1, 2020·Revista Argentina de microbiología·Cristian Javier MenaLaura Silvina Chiapello
Nov 13, 2010·Survey of Ophthalmology·Savitri SharmaSomasheila Murthy

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