PMID: 3754316Feb 14, 1986Paper

Detection of the mRNA coding for enkephalin precursor in the rat brain and adrenal by using an 'in situ' hybridization procedure

Neuroscience Letters
B BlochC Kowalski

Abstract

The messenger RNA coding for preproenkephalin A (PPA) has been detected in tissue sections of the rat brain and adrenal by using two rat PPA cDNAs labeled with 32P or 35S as probes. In the brain, neurons were labeled in areas known to correspond to sites of synthesis of enkephalins, including the caudate-putamen, the nucleus accumbens, the olfactory cortex, the hypothalamus, the brainstem and the granular layer of the cerebellum. The presence of the PPA mRNA in the normal rat adrenal medulla shows transcription of the PPA gene in such cells despite the absence of enkephalin immunoreactivity in them. These results demonstrate in situ hybridization as an efficient technique to detect the site of synthesis of PPA.

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B BlochA Kahn

❮ Previous
Next ❯

Citations

Jan 1, 1991·Brain Research. Molecular Brain Research·S CeccatelliT Hökfelt
Jan 1, 1994·Brain Research. Molecular Brain Research·I S ZagonP J McLaughlin
Jan 1, 1996·Brain Research. Molecular Brain Research·L JustD K Meyer
Feb 1, 1997·Brain Research. Molecular Brain Research·C Olenik, D K Meyer
Dec 3, 1999·Progress in Neuro-psychopharmacology & Biological Psychiatry·M S Cowen, A J Lawrence
Mar 1, 1997·Journal of Chemical Neuroanatomy·K V Gregg, G A Bishop
Jul 30, 1990·FEBS Letters·T PopoviciH Skala
Jun 1, 1990·The Journal of Comparative Neurology·S A Back, C Gorenstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.