Detection of the Novel optrA Gene Among Linezolid-Resistant Enterococci in Barcelona, Spain

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Jordi CàmaraM Ángeles Domínguez

Abstract

The purpose of this study was to describe the presence of the novel optrA gene among clinical isolates of enterococci in a Spanish teaching hospital (May 2016-April 2017). optrA and cfr genes were screened by PCR in all isolates showing linezolid minimal inhibitory concentration (MIC) ≥4 mg/L. The genetic relatedness of the isolates, the presence of resistance and virulence genes, and the genetic environment of optrA were assessed by whole-genome sequencing (WGS). Six of 1,640 enterococci had linezolid MIC ≥4 mg/L. Among them, the optrA gene was detected in five Enterococcus faecalis isolated from unrelated patients. Although none of them had received linezolid or chloramphenicol, all had antecedents of recent quinolone consumption. WGS analysis revealed the existence of two different genotypes: ST585 and ST474. cfr was not detected in any of the isolates. No mutations were detected among the 23S ribosomal RNA and the ribosomal proteins L3, L4, and L22. Both genotypes also carried genes related to aminoglycoside, lincosamide, macrolide, phenicol, and tetracycline resistance. Detection of optrA in a setting with low linezolid consumption and among patients without antecedents of oxazolidinone therapy is of concern.

References

Aug 22, 2000·Antimicrobial Agents and Chemotherapy·S SchwarzC Kehrenberg
Nov 10, 2006·Journal of Clinical Microbiology·Magdalena KawalecRob J L Willems
Jun 29, 2010·The Journal of Antimicrobial Chemotherapy·Victor E MulanovichP Rocco LaSala
Jun 2, 2014·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Rodrigo E MendesRonald N Jones
Sep 1, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J CaiJ Shen
Feb 3, 2016·Antimicrobial Agents and Chemotherapy·Robert K FlammRonald N Jones
Mar 24, 2016·MBio·Liam K R SharkeyAlex J O'Neill
Dec 22, 2016·The Journal of Antimicrobial Chemotherapy·Jinhu HuangLiping Wang
Feb 16, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I GawryszewskaE Sadowy
Jun 3, 2017·Journal of Global Antimicrobial Resistance·Veronika VorobievaAnette M Hammerum

❮ Previous
Next ❯

Citations

Apr 8, 2020·Antimicrobial Agents and Chemotherapy·Lara M AlmeidaMichael S Gilmore
Sep 25, 2020·Revista Panamericana De Salud Pública = Pan American Journal of Public Health·Sandra Yamile SaavedraCarolina Duarte
Feb 17, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M Angeles ArgudínM Hallin
Oct 23, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Heidrun KerschnerPetra Apfalter
Feb 21, 2021·Applied and Environmental Microbiology·Simona FioritiEleonora Giovanetti
May 7, 2021·Journal of Global Antimicrobial Resistance·Yamuna Devi BakthavatchalamBalaji Veeraraghavan
Apr 23, 2020·Enfermedades infecciosas y microbiología clínica·Alejandra AsenjoJuan-Ignacio Alós
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang
Jun 6, 2021·Enfermedades Infecciosas Y Microbiologia Clinica·Alejandra AsenjoJuan-Ignacio Alós
Nov 13, 2021·The Science of the Total Environment·Yang YuYa-Hong Liu

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP008816

Methods Mentioned

BETA
PCR

Software Mentioned

Geneious
Geneious assembler
Resfinder
NexteraXT
Virulence finder

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.