Detection of the quarantine species Thrips palmi by loop-mediated isothermal amplification

PloS One
Arnika PrzybylskaAleksandra Obrępalska-Stęplowska

Abstract

Thrips palmi (from the order Thysanoptera) is a serious insect pest of various crops, including vegetables, fruits and ornamental plants, causing significant economic losses. Its presence constitutes a double threat; not only does T. palmi feed on the plants, it is also a vector for several plant viruses. T. palmi originated in Asia, but has spread to North and Central America, Africa, Oceania and the Caribbean in recent decades. This species has been sporadically noted in Europe and is under quarantine regulation in the European Union. For non-specialists its larval stages are indistinguishable morphologically from another widespread and serious insect pest Frankliniella occidentalis (a non-quarantine species in the European Union) as well as other frequently occurring thrips. In this study, we have developed a loop-mediated isothermal amplification protocol to amplify rDNA regions of T. palmi. The results were consistent whether isolated DNA or crushed insects were used as template, indicating that the DNA isolation step could be omitted. The described method is species-specific and sensitive and provides a rapid diagnostic tool to detect T. palmi in the field.

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 9, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·A Obrepalska-SteplowskaJ Nawrot
Sep 10, 2009·Journal of Economic Entomology·K NowaczykJ Nawrot
Jul 21, 2012·Journal of Economic Entomology·Me-Chi YaoKuang-Hui Lu
Apr 2, 2014·Pest Management Science·Arnika JeszkeAleksandra Obrępalska-Stęplowska

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Citations

Feb 19, 2016·Archives of Virology·Marta BudziszewskaAleksandra Obrępalska-Stęplowska
Feb 9, 2019·Medical and Veterinary Entomology·C D SchenkelC Silaghi
Jul 9, 2017·Scientific Reports·Kaomud TyagiSumantika Chatterjee
Aug 28, 2021·Cells·Marianna SorokaAnna Rymaszewska

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Datasets Mentioned

BETA
KM877306
KM877308

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

GeneDoc
BLAST ( Basic Local Alignment Search Tool
MEGA4
PrimerExplorer
BioEdit
BLAST

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