PMID: 9418259Aug 1, 1997Paper

Detection of the Sec-1 locus of rye by a PCR-based method

Genes & Genetic Systems
Y ShimizuT R Endo

Abstract

The structural genes for the omega-secalins of rye (Secale cereale) are located in the Sec-1 locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the Sec-1 locus in a wheat genomic background. A primer set we designed based on a published sequence of a omega-secalin gene amplified not only the omega-secalin sequence, but also a putative omega-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the omega-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the Sec-1 locus in progeny of wheat lines carrying chromosome 1R.

References

Jan 1, 1988·Carlsberg Research Communications·J Entwistle
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard
Oct 1, 1995·Genome Génome / Conseil National De Recherches Canada·W BuschR Martin
Nov 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N K SinghR A McIntosh
Feb 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G G Doyle
Feb 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J SybengaP Shewry
Nov 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P R ShewryR P White
Dec 1, 1982·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J M FieldJ F March
Jan 1, 1981·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G J Lawrence, K W Shepherd

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Citations

Oct 28, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B HackaufP Wehling
Oct 18, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Tyson HowellJorge Dubcovsky
Apr 2, 2008·Genes & Genetic Systems·Masashi TsuchidaTakashi R Endo
Sep 21, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S R RouxP Wehling
Aug 15, 2002·Genome Génome / Conseil National De Recherches Canada·Ali Masoudi-NejadTakashi R Endo
Oct 15, 2019·Frontiers in Plant Science·Jens KeilwagenDragan Perovic
Oct 18, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Genqiao LiXiangyang Xu

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