Detection of the staphylococcal enterotoxin D-like gene from staphylococcal food poisoning isolates over the last two decades in Tokyo

The Journal of Veterinary Medical Science
Yasunori SuzukiYoichi Kamata

Abstract

The plasmid is a very well-known mobile genetic element that participates in the acquisition of virulence genes, such as staphylococcal enterotoxins (SEs), via horizontal transfer. SEs are emetic toxins and causative agents in staphylococcal food poisoning (SFP). We herein identified the types of plasmids harbored by seven SFP isolates and examined their production of plasmid-related SE/SEl to determine whether the new types of plasmid-related SE or SE-like (SEl) toxins (i.e. SElJ and SER) were involved in SFP. These isolates harbored pIB485-like plasmids, and all, except for one isolate, produced SElJ and SER. The amount of SER produced by each isolate accounted for the highest or second highest percentage of the total amount of SE/SEl produced. These new types of plasmid-related SE/SEls as well as classical SE may play a role in SFP. The seven isolates were classified into two SED-production types; a high SED-production type (>500 ng/ml) and no SED-production type. A nucleotide sequencing analysis revealed that three plasmids harbored by the SED-non-producing isolates had a single-base deletion in the sed gene with a resulting stop codon (from 233 amino acids of the intact SED to 154 amino acids of the mutant SED (mSED)). A r...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 27, 1990·Lancet·H S Tranter
Jan 11, 2000·Clinical Microbiology Reviews·M M DingesP M Schlievert
Mar 15, 2003·International Journal of Medical Microbiology : IJMM·Joseph E Alouf, Heide Müller-Alouf
Aug 23, 2005·Infection and Immunity·Katsuhiko OmoeKunihiro Shinagawa
Aug 20, 2008·Infection and Immunity·Hisaya K OnoKunihiro Shinagawa
Oct 8, 2008·Immunological Reviews·John D Fraser, Thomas Proft
Oct 20, 2009·Food Microbiology·Sylviane DerzelleVéronique Deperrois
Jan 12, 2010·Applied and Environmental Microbiology·Manon DuquenneAgnès Delacroix-Buchet
Jul 30, 2010·Cellular and Molecular Life Sciences : CMLS·Natalia Malachowa, Frank R DeLeo
Jul 1, 2010·Toxins·María Ángeles ArgudínMaría Rosario Rodicio
Nov 19, 2011·FEMS Microbiology Reviews·Jacques-Antoine HennekinneSylviane Dragacci
Jan 4, 2012·FEMS Immunology and Medical Microbiology·Hisaya K OnoKatsuhiko Omoe
Dec 21, 2012·Microbiology and Immunology·Yusuke Sato'oDong-Liang Hu
Jul 24, 2013·Infection and Immunity·Katsuhiko OmoeKen'ichi Imanishi
Nov 5, 2013·European Journal of Pharmacology·Dong-Liang Hu, Akio Nakane

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Citations

Apr 1, 2017·Critical Reviews in Food Science and Nutrition·Noreddine Benkerroum
Nov 18, 2017·Bioscience, Biotechnology, and Biochemistry·Shu YoshidaHiroaki Kanouchi
Sep 12, 2018·Infection and Immunity·Gillian J WilsonJ Ross Fitzgerald
Aug 19, 2017·Canadian Journal of Microbiology·Caroline Peixoto BastosWladimir Padilha da Silva
Aug 9, 2019·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yasunori Suzuki

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis

Software Mentioned

Genetyx
Statcel2
ORF Finder
Promega

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