Detection of the staphylococcal multiresistance gene cfr in Macrococcus caseolyticus and Jeotgalicoccus pinnipedialis

The Journal of Antimicrobial Chemotherapy
Yang WangJianzhong Shen

Abstract

To investigate the presence and the genetic environment of the multiresistance gene cfr in Jeotgalicoccus pinnipedialis and Macrococcus caseolyticus from pigs. A total of 391 bacterial isolates with florfenicol MICs ≥16 mg/L were obtained from nasal swabs of 557 individual pigs; of these, 75 Gram-positive isolates other than staphylococci and enterococci were screened by PCR for the presence of known florfenicol resistance genes. Species assignments of the cfr-carrying isolates were based on the results of biochemical profiling and 16S rDNA sequencing. The locations of the cfr gene were determined by Southern blotting. Regions flanking each cfr gene were sequenced by a modified random primer walking strategy, and the transferability of cfr was assessed by electrotransformation. Two M. caseolyticus isolates and one J. pinnipedialis isolate were cfr positive. The cfr gene was located either on a 7057 bp plasmid, pSS-03, which was widely distributed among staphylococci of pig origin, or on the ∼53 kb plasmid pJP1. The region of pJP1 that included the cfr gene and the adjacent IS21-558, showed 99.7% identity to the corresponding region of plasmid pSCFS3. In addition, the genes aadD + aacA-aphD, ble and erm(C), coding for aminoglyco...Continue Reading

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Dec 21, 2011·Antimicrobial Agents and Chemotherapy·Yang WangJianzhong Shen
Dec 29, 2011·Antimicrobial Agents and Chemotherapy·Yang LiuJianzhong Shen
Feb 15, 2012·The Journal of Antimicrobial Chemotherapy·Yang WangJianzhong Shen

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Citations

Apr 2, 2013·The Journal of Antimicrobial Chemotherapy·Jianzhong ShenStefan Schwarz
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Yang LiuJianzhong Shen
Jun 2, 2014·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Rodrigo E MendesRonald N Jones
Mar 31, 2015·Future Microbiology·Geovana Brenner MichaelStefan Schwarz
Nov 19, 2014·Antimicrobial Agents and Chemotherapy·Jian SunXiao-Ping Liao
Nov 3, 2016·Cold Spring Harbor Perspectives in Medicine·Stefan SchwarzBirte Vester
Nov 24, 2017·Animal Health Research Reviews·Yizhi TangQijing Zhang
Oct 16, 2016·Cold Spring Harbor Perspectives in Medicine·Susanne Paukner, Rosemarie Riedl
May 11, 2019·Microbiology Resource Announcements·Shahneela MazharOlivia McAuliffe
Feb 12, 2017·The Journal of Antimicrobial Chemotherapy·Yizhi TangQijing Zhang
Jun 28, 2019·The Journal of Antimicrobial Chemotherapy·Pattrarat ChanchaithongSybille Schwendener
Apr 22, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Liang-Xing FangXiao-Ping Liao
Jun 25, 2015·Microbiology Spectrum·Stefan SchwarzCong-Ming Wu
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang
Apr 7, 2018·Microbiology Spectrum·Zhangqi ShenJianzhong Shen

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