Detection of theileriosis in cattle and buffaloes by polymerase chain reaction

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
V R KundaveC G Joshi

Abstract

Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes.

References

Aug 1, 1982·Tropical Animal Health and Production·G UilenbergA A Spanjer
Jul 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·R TaharL K Basco
Mar 26, 1998·Tropical Animal Health and Production·C G Brown
Aug 6, 2000·International Journal for Parasitology·M J GubbelsF Jongejan
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Nicola E CollinsBasil A Allsopp
Oct 2, 2003·Veterinary Parasitology·G R RazmiM R Aslani
Dec 28, 2004·Veterinary Parasitology·N DumanliK Altay
Jun 13, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Saeid R Nourollahi-FardNima Ghalekhani

❮ Previous
Next ❯

Citations

Nov 24, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Hamidreza MajidianiDariush Saadati
Jun 13, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Leticia Gomes de PontesLucilene Delazari Dos Santos
Mar 6, 2021·Ticks and Tick-borne Diseases·Harshini Sheeja PrabhakaranManish Kumar
Jun 20, 2021·Acta Parasitologica·Aquil MohmadIshfaq Maqbool
Dec 7, 2021·Frontiers in Microbiology·Madhumanti BarmanParesh Sharma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.