Detection of Toxoplasma gondii in free-range, organic pigs in Italy using serological and molecular methods

International Journal of Food Microbiology
C BacciF Brindani

Abstract

Twenty-one free ranging pigs from three organically managed farms in northern Italy were examined for Toxoplasma gondii infection status by meat juice serology. DNA was extracted from all 21 animals and analysed for T. gondii by multilocus nested PCR-RFLP. Results showed a 95.2% prevalence in serology, while PCR was positive in 57.1% of infected pigs. Genotyping of amplified loci for Type I, Type II and Type I/II patterns, suggests the presence of more than one clonal genotype in circulation in these animals. Results of the present study highlight the high exposure to T. gondii in organic pig farms in Italy, indicating a potential risk for meat consumption.

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Citations

Dec 20, 2015·Foodborne Pathogens and Disease·Hai-Hai JiangQuan Liu
Aug 25, 2015·Experimental Parasitology·Qing YinXun Suo
Apr 26, 2016·International Journal of Food Microbiology·Sanata BambaStéphane Bretagne
Feb 17, 2017·Journal of Food Protection·Roberto PapiniAdriana Ianieri
Nov 4, 2017·Risk Analysis : an Official Publication of the Society for Risk Analysis·Roberto CondoleoZiad Mezher
Jul 19, 2018·Journal of Food Protection·Alberto VergaraGiovanni Normanno
Nov 9, 2018·Foodborne Pathogens and Disease·Jacek SrokaTomasz Cencek
Mar 11, 2019·Parasitology Research·Ana Carolina MiuraJoão Luis Garcia
Jun 27, 2019·Acta Veterinaria Hungarica·Ljiljana KurucaOlgica Djurković-Djaković
May 21, 2020·Parasites & Vectors·Tiziano IemmiNicola Ferrari
Feb 1, 2017·Parasitology Research·Anabela VilaresJoão Paulo Gomes
Aug 2, 2017·Foodborne Pathogens and Disease·Alberto De BerardinisAlberto Vergara
Dec 18, 2020·Frontiers in Veterinary Science·Mercedes Fernández-EscobarLuis Miguel Ortega-Mora
May 26, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xiao-Xuan ZhangHong-Bo Ni
Dec 4, 2020·Veterinary Parasitology·Jitender P DubeyChunlei Su

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