Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal

Journal of Agricultural and Food Chemistry
Ranjana SharmaTim A McAllister

Abstract

The persistence of plant-derived recombinant DNA in sheep and pigs fed genetically modified (Roundup Ready) canola was assessed by PCR and Southern hybridization analysis of DNA extracted from digesta, gastrointestinal (GI) tract tissues, and visceral organs. Sheep (n = 11) and pigs (n = 36) were fed to slaughter on diets containing 6.5 or 15% Roundup Ready canola. Native plant DNA (high- and low-copy-number gene fragments) and the cp4 epsps transgene that encodes 5-enolpyruvyl shikimate-3-phosphate synthase were tracked in ruminal, abomasal, and large intestinal digesta and in tissue from the esophagus, rumen, abomasum, small and large intestine, liver, and kidney of sheep and in cecal content and tissue from the duodenum, cecum, liver, spleen, and kidney of pigs. High-copy chloroplast-specific DNA (a 520-bp fragment) was detected in all digesta samples, the majority (89-100%) of intestinal tissues, and at least one of each visceral organ sample (frequencies of 3-27%) from sheep and swine. Low-copy rubisco fragments (186- and 540-bp sequences from the small subunit) were present at slightly lower, variable frequencies in digesta (18-82%) and intestinal tissues (9-27% of ovine and 17-25% of porcine samples) and infrequently in ...Continue Reading

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Citations

Mar 2, 2010·Applied Microbiology and Biotechnology·Matthias Brigulla, Wilfried Wackernagel
Oct 1, 2010·Animal : an International Journal of Animal Bioscience·R TudiscoF Infascelli
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·W Zhang, F Shi
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Nov 9, 2019·Journal of Animal Physiology and Animal Nutrition·Ahmad Ali ShahidTayyab Husnain

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