Detection of Trichophyton rubrum and Trichophyton interdigitale in onychomycosis using monoclonal antibodies against Sub6 (Tri r 2)

Mycoses
Bruno MéhulMichel Monod

Abstract

Onychomycosis is the most prevalent nail disease and is mainly caused by two dermatophyte species Trichophyton rubrum and Trichophyton interdigitale with a frequency in the range of 80% and 20%, respectively. The secreted protease Sub6 of the subtilisin family, which was never detected in vitro growth conditions, was found to be a robust marker of onychomycosis. The aim of this work was to detect tinea unguium using anti-Sub6 monoclonal antibodies in proteins extracted from clinical nail samples. We produced monoclonal antibodies in mice using recombinant Sub6 as an antigen. Selected monoclonal antibodies were tested by Western blot analysis and ELISA on protein extracts from onychomycosis samples. Several monoclonal antibodies used to quantify Sub6 in proteins extracted from clinical nail samples were produced and characterised. We showed that these antibodies were very specific and allowed the detection of T. rubrum and T. interdigitale in onychomycosis. Sub6 was detected in clinical samples infected by T. rubrum and not detected in nails with trauma and other diseases. Anti-Sub6 monoclonal antibodies could be useful for a rapid diagnosis of tinea unguium and/or therapeutic survey of dermatophyte in onychomycosis by ELISA or ...Continue Reading

References

Sep 10, 2002·Dermatology : International Journal for Clinical and Investigative Dermatology·Michael MonodRenato Panizzon
Sep 15, 2004·Gene·Olivier JoussonMichel Monod
Mar 23, 2012·International Journal of Dermatology·Yuko HigashiTakuro Kanekura
Aug 3, 2016·Mycopathologia·Julie Verrier, Michel Monod

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Citations

Mar 1, 2019·Journal of Fungi·Michel Monod, Bruno Méhul
Dec 22, 2020·Frontiers in Immunology·Verónica L BursteinLaura S Chiapello

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