Detection of trisomy 12 and centromeric alterations in CLL by interphase- and metaphase-FISH

Cancer Genetics and Cytogenetics
H Acar, M J Connor

Abstract

We have studied trisomy 12 in chronic lymphocytic leukemia (CLL) by fluorescence in situ hybridization (FISH) with an alpha-satellite centromeric probe for chromosome 12 on both dividing and non-dividing cells. Trisomy for chromosome 12 was demonstrated in four of these patients (15.3%) using FISH on interphase cells. The percentage of trisomic cells ranged from 10% to 65% of nuclei. The hybridization signals in the trisomic and disomic nuclei were of a broadly similar size and nature. Interestingly, three of the remaining CLL patients, who exhibited disomy for chromosome 12, showed a marked difference in size of the hybridization signals in interphase nuclei. This was also demonstrated in metaphase spreads. In addition, metaphase FISH studies revealed a supernumerary marker chromosome in three out of 26 patients with CLL.

References

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Citations

May 29, 1999·Cancer Genetics and Cytogenetics·A D Stock, T R Dennis
Oct 14, 1998·Trends in Genetics : TIG·J Hodgkin, R K Herman
Dec 1, 2007·Advances in Anatomic Pathology·Bharat N NathwaniMilton R Drachenberg
Jul 10, 1999·Current Opinion in Hematology·W G Wierda, T J Kipps

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