Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction.

Journal of Clinical Microbiology
D R MoserJ E Donelson

Abstract

The polymerase chain reaction was used to amplify a 188-base pair (bp) segment of the repetitive 195-bp nuclear DNA sequence of Trypanosoma cruzi that is the most abundant sequence in this organism. The reaction amplified this repetitive element in four T. cruzi isolates from widely separated geographic regions. No amplification of the 188-bp fragment occurred when DNAs extracted from Leishmania spp., African trypanosomes, or blood samples from mice and humans were used. Amplification of one-half of the DNA from a single T. cruzi parasite produced an amount of the 188-bp element that was readily visible in a gel stained with ethidium bromide. Hybridization of a radiolabeled probe to membrane-bound amplification products increased the sensitivity to a level at which 1/200 of the DNA in a single parasite could be detected. T. cruzi DNA was readily detected in DNA extracted from the abdominal contents of infected insect vectors reared in the laboratory. No parasite DNA was detected in the blood samples of two individuals known to be infected with T. cruzi, possibly because in such patients the number of circulating parasites are extremely low or because parasitemias are intermittent. These results represent a considerable increase...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1987·Molecular and Biochemical Parasitology·G A Cook, J E Donelson
Jan 1, 1988·Molecular and Biochemical Parasitology·W DegraveC M Morel
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W O Rogers, D F Wirth
May 1, 1987·The American Journal of Medicine·L V KirchhoffF C Gilliam
Mar 1, 1988·Journal of Clinical Microbiology·F AshallM A Miles
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A LakeL Simpson
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Mar 12, 1987·The New England Journal of Medicine·S H EmburyH A Erlich
Jan 1, 1973·Annual Review of Microbiology·Z Brener
Sep 1, 1981·Molecular and Biochemical Parasitology·D E LanarJ E Manning
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jun 15, 1983·Journal of Molecular Biology·P SloofW Leon
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A GonzalezP M Lizardi
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M J LenardoJ E Donelson
Feb 1, 1984·The Journal of protozoology·W J MurphyJ E Donelson
Oct 15, 1955·Journal of the American Medical Association·N C WOODY, H B WOODY

❮ Previous
Next ❯

Citations

Apr 1, 1992·Neuropathology and Applied Neurobiology·S Love, J A Nicoll
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·W L Tafuri
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·A O Luquetti
Feb 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·María Flores-ChávezCarmen Cañavate
Feb 2, 2010·Emerging Infectious Diseases·C Miguel PintoRobert D Bradley
Jan 1, 2012·ISRN Veterinary Science·Luciano José Eloy, Simone Baldini Lucheis
Aug 30, 2014·Journal of Biomolecular Screening·Alessandra Ricciardi, Momar Ndao
Dec 19, 2014·PLoS Neglected Tropical Diseases·Maria C GuimaroAntonio R L Teixeira
Jan 23, 2016·PLoS Neglected Tropical Diseases·Lisa A ShenderJonna A K Mazet
Jan 23, 2016·Parasite : Journal De La Société Française De Parasitologie·Jack-Yves DeschampsFrançoise A Roux
Nov 2, 2016·American Journal of Primatology·Michele BahiaAlessandra Scofield
Jan 1, 1992·Revista Da Sociedade Brasileira De Medicina Tropical·G Brasileiro Filho, S D Pena
Feb 1, 1991·The Journal of Applied Bacteriology·K WernarsS H Notermans
Jan 18, 1990·The New England Journal of Medicine·B I Eisenstein
Jun 6, 2002·Memórias do Instituto Oswaldo Cruz·Simone Frédérique BrenièreMireille Hontebeyrie
Nov 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·M A Shikanai-YasudaL V Kirchhoff
Jul 30, 2003·Memórias do Instituto Oswaldo Cruz·Joshua E LaneClint E Carter
Jun 24, 2004·Infection and Immunity·Kara L Cummings, Rick L Tarleton
Jan 2, 2001·Journal of the American Veterinary Medical Association·K K BradleyL V Kirchhoff
Mar 30, 2006·Parasitology Research·Ana Maria de CastroLúcia Maria da Cunha Galvão
Feb 13, 2007·Memórias do Instituto Oswaldo Cruz·Augusto Simões-BarbosaAntonio R L Teixeira
Apr 7, 2011·PLoS Neglected Tropical Diseases·Antonio R L TeixeiraMariana M Hecht
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Constança Carvalho Britto
Jun 9, 2009·PLoS Neglected Tropical Diseases·Stijn DeborggraevePhilippe Büscher
Nov 25, 2014·Emerging Infectious Diseases·Etienne WaleckxPatricia L Dorn
Feb 26, 2014·The American Journal of Tropical Medicine and Hygiene·James N MubiruJohn L VandeBerg
Jan 28, 2016·Parasites & Vectors·Thaís Tâmara Castro Minuzzi-SouzaRodrigo Gurgel-Gonçalves
May 11, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Andressa Noronha Barbosa-SilvaLúcia Maria da Cunha Galvão
Apr 22, 2020·Infection and Immunity·Bijay K JhaBradford S McGwire
Nov 1, 1992·Die Naturwissenschaften·R Felleisen, M Q Klinkert
May 7, 2002·Memórias do Instituto Oswaldo Cruz·María Esther BarCristina Wisnivesky-Colli
Jun 11, 1999·The Journal of Eukaryotic Microbiology·E E MontamatA Blanco

❮ Previous
Next ❯

Related Concepts

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A GonzalezP M Lizardi
Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
S M GwaltneyR D Oberst
© 2022 Meta ULC. All rights reserved